| Автор | Dumitru, loana |
| Автор | McNeil, J.Bryan |
| Дата выпуска | 1994 |
| dc.description | In this report a modification to the in vivo footprinting assay is described. The method includes dimethyl sulfate treatment of whole yeast cells, followed by reiterative primer extension of the methylated genomic DNA using Taq DNA polymerase. Under appropriate reaction conditions chain extension terminates opposite a methylated purlne when Taq DNA polymerase encounters a modified adenlne or guanine. The procedure was used to examine, in vivo DNA-protein contacts over the upstream activation site (UAS) of the Saccharomyces cerevlslae PYK gene. in vivo analysis, using isogenic strains of yeast and Escherichla coli transformed with plasmid DNAs, confirmed the binding of both the frens-actlng factor RAP1 and the transcriptlonal activator GCR1 to cis-acting recognition sites located within the PYK UAS element. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © Oxford University Press |
| Тема | MOLECULAR BIOLOGY |
| Название | A simple in vivo footprinting method to examine DNA-protein interactions over the yeast PYK UAS element |
| Тип | other |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 22 |
| Первая страница | 1450 |
| Последняя страница | 1455 |
| Аффилиация | Department of Microbiology, University of TorontoToronto, Ontario M5S 1A8, Canada |
| Выпуск | 8 |
