| Автор | Kovi, Joseph |
| Автор | Kovi, Elizabeth |
| Автор | Morris, Harold P. |
| Автор | Rao, Mamidanna S. |
| Дата выпуска | 1978 |
| dc.description | AbstractThe chromosome constitution of transplantable hepatomas H-35tc<sub>1</sub>, 7316A, and 8994 induced in ACI male, BUF female, and BUF male rats, respectively, by monocyclic or polycyclic aromatic amines was studied by Giemsa banding techniques. Hepatoma H-35tc<sub>1</sub> was hyperdiploid, with a dominant stemline of 46 chromosomes. The stemline of the heterogeneous 7316A was in the hypotetraploid region (75–82). Hepatoma 8994 had a near-triploid complement; most metaphase cells had chromosome numbers from 62 to 68. Thirty marker chromosomes were detected. Nineteen rearranged chromosomes were in hepatoma 8994, whereas only 8–10 markers could be found in the 80±3 chromosome complement of hepatoma 7316A. Two constant common markers were noted: mar2, a chromosome #11 with translocation short arm in H-35tc<sub>1</sub> and 8994, and mar10 (mar10a), a chromosome #1 with a duplicated segment at breakpoint q54 in hepatomas 7316A and 8994. An analysis of the distribution of 232 breakpoints in the rat karyotype, 26 identified in the hepatomas and 206 collected from the literature, revealed a statistically significant excess of breaks in chromosomes #1, 2, 3, and 10 (P<0.001). The X- and Y-chromosomes showed a considerably lower number of breaks than anticipated (P<0.01). Even after a history of continuous transplantation for 10 years, these 3 hepatomas retained intact sex chromosomes that corresponded to the phenotype of the primary host. Preservation of the sex chromosomes in the hepatomas may be attributed to a lower susceptibility of the sex chromosomes than of the autosomes to breakage. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Тема | Investigations on Nonhuman Systems |
| Название | Chromosome Banding Patterns and Breakpoints of Three Transplantable Hepatomas Induced in Rats by Aromatic Amines23 |
| Тип | research-article |
| DOI | 10.1093/jnci/61.2.495 |
| Electronic ISSN | 1460-2105 |
| Print ISSN | 0027-8874 |
| Журнал | JNCI: Journal of the National Cancer Institute |
| Том | 61 |
| Первая страница | 495 |
| Последняя страница | 506 |
| Аффилиация | Kovi Joseph; Department of Pathology, Howard University College of Medicine |
| Аффилиация | Kovi Elizabeth; Department of Biochemistry, Howard University College of Medicine |
| Аффилиация | Morris Harold P.; Department of Biochemistry, Howard University College of Medicine |
| Аффилиация | Rao Mamidanna S.; Department of Community Health and Family Practice, Howard University College of Medicine |
| Выпуск | 2 |
