| Автор | Yagi, Mary Jane |
| Автор | Maldarelli, Frank |
| Автор | Chin, Susan E. |
| Автор | Holland, James F. |
| Автор | Bekesi, J. George |
| Дата выпуска | 1986 |
| dc.description | PTT.119 [p-fluoro-l-Phe-m-bis-(2-chloroethyl)-amino-l-Phe-Met ethoxy HCl] is a new bifunctional alkylating compound that possesses both cytolytic and antiviral activities. Continuous treatment of mouse mammary tumor cells with 15 µM PTT.119 reduced production of the B-type retrovirus murine mammary tumor virus (MuMTV). MuMTV levels in PTT.119-treated cultures were reduced by 31% in the first 24 hours; an additional 24 hours of treatment resulted in a further reduction of 70%. These reductions were significantly greater than could be accounted for by the effects of PTT.119 tumor cell metabolism and viability. Under identical treatment conditions, equimolar concentrations of L-phenylalanine mustard reduced MuMTV production by only 3%. PTT.119 inhibition of MuMTV replication was also apparent when mammary tumor cells were exposed for periods as short as 0.5–4 hours; persistent decreases in virus production were detected even 7 days following tripeptide treatment. Analyses of the polypeptide composition of MuMTV by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that virions from these PTT.119-treated mammary tumor cultures contained significant reductions in the relative level of the nonglycosylated 24,000-dalton (p24) viral polypeptide associated with the nucleoprotein core. Decreases in p24 were observed in MuMTV produced in the presence of the tripeptide and 1–7 days after removal of PTT.119. Examination of the steady-state levels and rates of intracellular MuMTV protein synthesis suggested that PTT.119 interferes with late steps in MuMTV processing and maturation. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Тема | Investigations on Nonhuman Systems |
| Название | Interference of Mouse Mammary Tumor Virus Replication by PTT.119 [p-Fluoro-l-Phe-m-bis-(2-chloroethyl)amino-l-Phe-Met ethoxy HCl]—an Antitumor Agent2 |
| Тип | research-article |
| DOI | 10.1093/jnci/76.3.493 |
| Electronic ISSN | 1460-2105 |
| Print ISSN | 0027-8874 |
| Журнал | JNCI: Journal of the National Cancer Institute |
| Том | 76 |
| Первая страница | 493 |
| Последняя страница | 501 |
| Выпуск | 3 |
