| Автор | Gallie, Daniel R. |
| Автор | Sleat, David E. |
| Автор | Watts, John W. |
| Автор | Turner, Philip C. |
| Автор | Wilson, T.Michael A. |
| Дата выпуска | 1988 |
| dc.description | Mutational analysis of the 5′-untranslated leader sequence (Ω) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with Ω. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alterations in Ω's ability to enhance expression of β-glucuronidase (GUS) RNA in tobacco mesophyll protoplasts and Escherithia coli or chioramphenicol acetyltransferase (CAT) RNA in Xenopus laevis oocytea. Alteration of an eight base subsequence required for the binding of a second ribosoe resulted in the loss of translational enhancement in X. laevis oocytes but not in protoplasts. Substantial increases in enhancement were observed for several of the utants in E. coli. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © IRL Press Limited |
| Тема | Articles |
| Название | Mutational analysis of the tobacco mosaic virus 5′-leader for altered ability to enhance translation |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 16 |
| Первая страница | 883 |
| Последняя страница | 893 |
| Аффилиация | Department of Virus ReseachColney Lane, Norwich NR4 7UH |
| Аффилиация | Watts John W.; Cell Biology, John Innes Institute, AFRC Institute of Plant Science Research |
| Аффилиация | Turner Philip C.; Department of Biochemistry, University of Liverpool |
| Аффилиация | Wilson T.Michael A.; Cell Biology, John Innes Institute, AFRC Institute of Plant Science Research |
| Выпуск | 3 |
