| Автор | Takao, Masashi |
| Автор | Abramic, Marija |
| Автор | Moos, Malcolm |
| Автор | Rapic' Otrin, Vesna |
| Автор | Wootton, John C. |
| Автор | McLenigan, Mary |
| Автор | Levine, Arthur S. |
| Автор | Protic, Miroslava |
| Дата выпуска | 1993 |
| dc.description | A cDNA which encodes á 127 kDa UV–damaged DNA–binding (UV-DDB) protein with high affinity for (6—4)pyrlmidine dimers [Abramic', M., Levine, A.S. & Protic', M., J. Biol. Chem. 266:22493—22500,1991] has been Isolated from a monkey cell cDNA library. The presence of this protein in complexes bound to UV-damaged DNA was confirmed by immunobiotting. The human cognate of the UV-DDB gene was localized to chromosome 11. UV-DDB mRNA was expressed in all human tissues examined, including cells from two patients with xeroderma pigmentosum (group E) that are deficient in UV-DDB activity, which suggests that the binding defect in these cells may reside in a dysfunctional UV-DDB protein. Database searches have revealed significant homology of the UV-DDB protein sequence with partial sequences of yet uncharacterized proteins from Dictyostellum discoldeum (44% Identity over 529 amino acids) and Oryza satlva (54% identity over 74 residues). According to our results, the UV-DDB polypeptide belongs to a highly conserved, structurally novel family of proteins that may be involved in the early steps of the UV response, e.g., DNA damage recognition. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1993 Oxford University Press |
| Тема | MOLECULAR BIOLOGY |
| Название | A 127 kDa component of a UV-damaged DNA-binding complex, which is defective in some xeroderma pigmentosum group E patients, is homologous to a slime mold protein |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 21 |
| Первая страница | 4111 |
| Последняя страница | 4118 |
| Аффилиация | Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human DevelopmentBethesda, MD 20892, USA |
| Аффилиация | Moos Malcolm; Laboratory of Developmental Biology, Division of Cellular and Gene Therapy, Food and Drug Administration |
| Аффилиация | Wootton John C.; National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health |
| Выпуск | 17 |
