| Автор | Shaw, Jeng-Pyng |
| Автор | Kent, Kenneth |
| Автор | Bird, Jeff |
| Автор | Fishback, James |
| Автор | Froehler, Brian |
| Дата выпуска | 1991 |
| dc.description | Unmodified deoxyoligonucleotides are rapidly degraded in serum-containing medium. Utilizing internally labelled deoxyoligonucleotides the deoxyribonuclease profile for fetal calf serum and human serum was determined. It was found that the predominate nuclease activity in both systems was 3' exonuclease. Deoxyoilgonucleotides are protected from exonuclease degradation In sera and cell media by simple terminal modifications that maintain high binding affinity for the complementary DNA sequence. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1991 Oxford University Press |
| Тема | CHEMISTRY |
| Название | Modified deoxyoligonucleotides stable to exonuclease degradation in serum |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 19 |
| Первая страница | 747 |
| Последняя страница | 750 |
| Аффилиация | Gilead Sciences346 Lakeside Drive, Foster City, CA 94404, USA |
| Выпуск | 4 |
