| Автор | Lepault, Jean |
| Автор | Bram, Stanley |
| Автор | Escaig, Jacques |
| Автор | Wray, Wayne |
| Дата выпуска | 1980 |
| dc.description | Chromatin gels, metaphase chromosomes, and intact nuclei were studied by freeze fracturing followed by electron microscopy. The results complement and extend those obtained by classical electron microscopy techniques as they are obtained without fixation or dehydration. The freeze fracturing technique permits a determination of the hydrated diameters of nucleosanes in chromatin and in nuclei to be 13 nm by comparing to simultaneously studied test objects. Nucleosomes in chromatin fibers are closely spaced but are discrete particles in all conditions studied. In the presence of divalent ions, most chromatin in solution, chromosomes, and nuclei is organized into fibers whose thickness is larger than 40 nm. The images are not at all compatible with a super bead organization of the nucleofilament. Freeze fractures of intact nuclei provides information on the distribution of chromatin in a hydrated unfixed state. The images suggest that most of the chromatin is localized in large domains in contact with the inner nuclear membrane. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © IRL Press Limited. |
| Тема | Article |
| Название | Chromatin freeze fracture electron microscopy: a comparative study of core particles, chromatin, metaphase chromosomes, and nuclei |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 8 |
| Первая страница | 265 |
| Последняя страница | 278 |
| Аффилиация | Lepault Jean; Institut Pasteur, |
| Аффилиация | Bram Stanley; Institut Pasteur, |
| Аффилиация | Escaig Jacques; Laboratoire de Microscopie Electronique Appliquéa la Biologie, Université de Paris IV, |
| Аффилиация | Wray Wayne; Department of Cell Biology, Baylor College of Medicine, |
| Выпуск | 2 |
