| Автор | Nishida, Toshirou |
| Автор | Inoue, Toru |
| Автор | Kamiike, Wataru |
| Автор | Kawashima, Yasunaru |
| Автор | Tagawa, Kunio |
| Дата выпуска | 1989 |
| dc.description | During anoxic incubation, depletion of mitochondrial ATP was followed by release of Ca<sup>2+</sup> with concomitant increase in the rate of state 4 respiration due to disruption of the diffusion barrier against protons. The external addition of ATP and its non-metabolizable analog, β, γ-methylene adenosine 5′-triphosphate, prevented both the release of Ca<sup>2+</sup> and increase in the rate of state 4 respiration. Addition of EGTA, which did not prevent release of the ion, resulted in little increase in the respiration rate. Addition of an inhibitor of mitochondrial phospholipase A<sub>2</sub>, such as quinacrine, dibucaine, or chlorpromazine, also prevented increase in the respiration rate without affecting Ca<sup>2+</sup> release from mitochondria during anoxic incubation. Non-esterified polyunsaturated fatty acids were also found to be liberated from anoxic mitochondria. External addition of the ATP-analog, EGTA, and inhibitors of phospholipase A<sub>2</sub> suppressed the liberation of non-esterified polyunsaturated fatty acids. Melittin and Ca<sup>2+</sup>, which activate phospholipase A<sub>2</sub>, increased the rate of state 4 respiration and the liberation of fatty acids. These findings support the hypothesis proposed previously that the following sequence changes occurs in mitochondria during anoxia; depletion of ATP, liberation of free calcium from mitochondria, and disruption of the diffusion barrier against H<sup>+</sup> of the inner membrane. The results also indicate another event; activation of phospholipase A<sub>2</sub> by release Ca<sup>2+</sup> which results in H<sup>+</sup> leakiness of the inner membrane. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1989 BY THE JOURNAL OF BIOCHEMISTRY |
| Тема | REGULAR PAPERS |
| Название | Involment of Ca<sup>2+</sup> Release and Activation of Phospholipase A<sub>2</sub> in Mitochondrial Dysfuntion during Anoxia<sup>1</sup> |
| Тип | research-article |
| Electronic ISSN | 1756-2651 |
| Print ISSN | 0021-924X |
| Журнал | Journal of Biochemistry |
| Том | 106 |
| Первая страница | 533 |
| Последняя страница | 538 |
| Аффилиация | Nishida Toshirou; First Department of Surgery, Medical School, Osaka University |
| Аффилиация | Inoue Toru; Department of Physiological Chemistry, Osaka University |
| Аффилиация | Kamiike Wataru; First Department of Surgery, Medical School, Osaka University |
| Аффилиация | Kawashima Yasunaru; First Department of Surgery, Medical School, Osaka University |
| Аффилиация | Tagawa Kunio; Department of Physiological Chemistry, Osaka University |
| Выпуск | 3 |
