| Автор | Reithmann, C. |
| Автор | Gierschik, P. |
| Автор | Jakobs, K. H. |
| Автор | Werdan, K. |
| Дата выпуска | 1991 |
| dc.description | The regulation of adenylyl cyclase components and of adenylyl cyclase activity by noradrenaline and tumour necrosis factor α (TNFα) was studied in rat cardiomyocytes. Long-term treatment of rat cardiomyocytes in the presence of noradrenaline leads, in addition to a down-regulation of β<sub>1</sub>-adrenoceptors, to an increase in the level of inhibitory G protein α-subunits and to a heterologous descnsitization of adenylyl cyclase stimulation. Similar to the noradrenaline exposure, incubation of the cardiomyocytes in the presence of the cytokine TNFα (10 U. ml<sup>−1</sup>) also increases the level of G<sub>1α</sub> proteins. However, in contrast to the noradrenaline treatment, which apparently induces a selective up-regulation of G<sub>1α</sub>, the TNFα exposure also increases the level or activity of other components of adenylyl cyclase, such as the level of membrane β<sub>36</sub>-subunits of G proteins and, most likely, the level of the α-subunits of the stimulatory G protein (G<sub>sα</sub>) and the activity of adenylyl cyclase catalytic subunit. While noradrenaline treatment desensitizes receptor-dependent and independent adenylyl cyclase activity, treatment of the cells with TNFα induces a sensia'zation of adenylyl cyclase stimulation.The data indicate that only a selective increase in the level of inhibitory G protein a subunits decreases adenylyl cyclase activity. The hypersensitivity of adenylyl cyclase induced by TNFα exposure may be due to concomitant alterations of other components of the adenylyl cyclase signal transduction system. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1991 The European Society of Cardiology |
| Тема | Adenylyl cyclase |
| Тема | G proteins |
| Тема | noradrenaline |
| Тема | tumour necrosis factor α |
| Тема | Articles |
| Название | Regulation of adenylyl cyclase by noradrenaline and tumour necrosis factor α in rat cardiomyocytes |
| Тип | research-article |
| DOI | 10.1093/eurheartj/12.suppl_F.139 |
| Electronic ISSN | 1522-9645 |
| Print ISSN | 0195-668X |
| Журнал | European Heart Journal |
| Том | 12 |
| Первая страница | 139 |
| Последняя страница | 142 |
| Аффилиация | Medizinische Klinik I, Universität München, Klinikum GroBhadernD-8000 München 70, Marchioninistr. 15 |
| Аффилиация | Gierschik P.; Pharmakologisches Institut, Universität Heidelberg |
| Аффилиация | Jakobs K. H.; Pharmakologisches Institut, Universität Heidelberg |
| Выпуск | suppl_F |
