| Автор | DEANE-DRUMMOND, CELIA E. |
| Дата выпуска | 1984 |
| dc.description | The effects of Cl<sup>−</sup> and NO3- pretreatment on <sup>36</sup>Cl<sup>−</sup>influx and ClO3-/NO3- influx into Chara corallina cells were examined. Both treatments reduced <sup>36</sup>Cl<sup>−</sup> influx into C. corallina cells in the acid pH range (4.5–7.0). NO3- pretreatment stimulated /NO3- influx into C. corallina cells, but Cl<sup>−</sup> pretreatment had no effect. There was a direct inhibitory effect of CI on ClO3-/NO3- influx into C. corallina cells, but no apparent effect on net NONO3- uptake.The time course of <sup>36</sup>Cl accumulation into cytoplasmic and vacuolar compartments during incubation of the cells with ClO3- showed that significant radioactivity appeared in the vacuolar sap after 30 min. There was a linear increase in the percentage of total <sup>36</sup>CI which crossed the tonoplast (φ<sub>c-v</sub>). There was no effect of Cl<sup>−</sup> or NO3- pretreatment on accumulation of radioactivity in the vacuole. Thin layer chromatography of the vacuolar sap showed that after 2 h only one component was present which had an R<sub>F</sub> which was similar to <sup>36</sup>CI<sup>−</sup>. The rate of accumulation of <sup>36</sup>C1<sup>−</sup> in the vacuole could be used to estimate rates of ClO3- reduction. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © Oxford University Press |
| Тема | Chloride |
| Тема | Chlorate |
| Тема | Chara |
| Тема | Nitrate |
| Тема | Articles |
| Название | Nitrate Transport into Chara corallina Cells using ClO3-as an Analogue for Nitrate |
| Тип | research-article |
| Electronic ISSN | 1460-2431 |
| Print ISSN | 0022-0957 |
| Журнал | Journal of Experimental Botany |
| Том | 35 |
| Первая страница | 1733 |
| Последняя страница | 1743 |
| Аффилиация | Botany SchoolDowning Street, Cambridge CB2 3EA, U.K |
| Выпуск | 12 |
