The nucleotide sequence of Euglena cytoplasmic phenylalanine transfer RNA. Evidence for possible classification of Euglena among the animal rather than the plant kingdom
Chang, S.H.; Hecker, L.I.; Brum, C.K.; Schnabel, J.J.; Heckman, J.E.; Silberklangt, M.; Raj-Bhandary, U.L.; Barnett, W.E.; Chang S.H.; Department of Biochemistry, Louisiana State University; Hecker L.I.; Biology Division, Oak Ridge National Laboratory; Brum C.K.; Department of Biochemistry, Louisiana State University; Schnabel J.J.; Department of Biochemistry, Louisiana State University; Heckman J.E.; Department of Biology, Massachusetts Institute of Technology; Silberklangt M.; Department of Biology, Massachusetts Institute of Technology; Raj-Bhandary U.L.; Department of Biology, Massachusetts Institute of Technology; Barnett W.E.; Biology Division, Oak Ridge National Laboratory
Журнал:
Nucleic Acids Research
Дата:
1981
Аннотация:
The nudeotide sequence of cytoplasmic phenylalanine tRNA from Euglena gracilis has been elucidated using procedures described previously for the corresponding chloroplastic tRNA [Cell, 9, 717 (1976)]. The sequence is:pG-C-C-G-A-C-U-U-A-m<sup>2</sup>G-C-U-Cm-A-G-D-D-G-G-G-A-G-A-G-C-m<sub>2</sub><sup>2</sup>G-ψ-ψ-A-G-A-Cm-U-Gm-A-A-Y-A-ψ-C-U-A-A-A-G-m<sup>7</sup>G-U-C-*C-C-U-G-G-T-ψ-C-G-m<sup>1</sup>A-U-C-C-C-G-G-G-A-G-ψ-CG-G-C-A-C-C-A. Like other tRNA<sup>Phe<sub>s</sub></sup> thus far sequenced, this tRNA has achain length of 76 nucleotides. The sequence of E. gracilis cytoplasmic tRNA<sup>phe</sup> is quite different (27 nucleotides out of 76 different) from that of the corresponding chloroplastic tRNA but is surprisingly similar (72 out of 76 nucleotides identical) to that of tRNA<sup>phe</sup> from mammalian cytoplasm. This extent of sequence homology even exceeds that found between 12. gracilis and wheat germ cytoplasmic tRNA<sup>phe</sup>. These findings raise interesting questions on the evolution of tRNAs and the taxonomy of Euglena.
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