| Автор | Mitsuoka, Wataru |
| Автор | Egashira, Shogo |
| Автор | Tagawa, Hirofumi |
| Автор | Kuga, Takeshi |
| Автор | Hayashi, Yasuo |
| Автор | Yamada, Akira |
| Автор | Tomoike, Hitonobu |
| Автор | Nakamura, Motoomi |
| Автор | Takeshita, Akira |
| Дата выпуска | 1995 |
| dc.description | AbstractObjective: X-irradiation is known to enhance atherosclerotic change. We tested whether coronary vasoconstrictor responses are augmented at the sites of X-ray-induced intimal thickening in Göttingen miniature pigs. Methods: In 17 pigs, a major branch of the left coronary artery was denuded with a balloon catheter. In 10 pigs, the denuded portion of the left coronary artery was selectively irradiated with 15 Gy of X-rays twice at 3 and 4 months after denudation (group 1). The remaining 7 pigs were not irradiated (group 2). The effects of intracoronary administration of serotonin, histamine and phenylephrine on the coronary diameter were studied 3 (3M) and 5 months (5M) after denudation. After the angiographical study at 5M, the vessels were isolated and isometric tension was measured in an organ chamber. Results: The percent reduction in coronary diameter evoked with 10 μg · kg<sup>−1</sup> of serotonin increased from 39(s.e.m. 4)% before X-irradiation (3M) to 75(6)% after X-irradiation (5M) in group 1 (P < 0.01), while it did not differ in group 2 [39(6)% at 3M vs. 33(8)% at 5M]. In group 1, serotonin-induced coronary constriction was frequently accompanied by ischemic ECG changes. Histamine (10 μg · kg<sup>−1</sup>)-induced vasoconstriction was also augmented but to a smaller degree [47(6)% at 3M vs. 62(4)% at 5M; P < 0.05] in group 1, while it remained unchanged in group 2 [52(5)% at 3M vs. 44(7)% at 5M]. Phenylephrine did not cause detectable contraction in either group at 3M or 5M. Methysergide and ketanserin attenuated serotonin-induced hypercontraction in a dose-dependent fashion. In the in vitro studies, endothelium-dependent relaxation to serotonin was impaired at the denuded site with (group 1) and without (group 2) X-irradiation to a similar extent. Isometric tension of medial smooth muscle developed by serotonin was significantly greater at the denuded site with X-irradiation (group 1) than the control site and the denuded site without X-irradiation (group 2) (P < 0.05). Intimal thickening was significantly greater at the denuded sites with X-irradiation [group 1, 238(45) μm] than at the denuded sites without X-irradiation [group 2, 58(5) μm] (P < 0.05). Conclusions: These results indicate that X-irradiation augments the coronary vasoconstrictor responses to autacoids, predominantly to serotonin, and that this augmentation is accompanied by enhanced intimal thickening. Serotonin-induced hypercontraction after X-irradiation resulted mainly from the hyperreactivity of medial smooth muscle. |
| Формат | application.pdf |
| Издатель | Elsevier Science |
| Копирайт | Copyright © 1995, European Society of Cardiology |
| Тема | X-ray |
| Тема | Coronary artery tone |
| Тема | Serotonin |
| Тема | Histamine |
| Тема | Atherosclerosis |
| Тема | Pig coronary arteries |
| Тема | Hyperreactivity |
| Тема | Endothelium-dependent relaxation |
| Тема | Original Article |
| Название | Augmentation of coronary responsiveness to serotonin at the site of X-ray-induced intimal thickening in miniature pigs |
| Тип | research-article |
| DOI | 10.1016/S0008-6363(95)00038-0 |
| Electronic ISSN | 1755-3245 |
| Журнал | Cardiovascular Research |
| Том | 30 |
| Первая страница | 246 |
| Последняя страница | 254 |
| Аффилиация | Mitsuoka Wataru; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Egashira Shogo; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Tagawa Hirofumi; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Kuga Takeshi; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Hayashi Yasuo; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Yamada Akira; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Аффилиация | Tomoike Hitonobu; <sup>b</sup>First Division of Internal Medicine, Faculty of Medicine, Yamagata University, Yamagata, Japan |
| Аффилиация | Nakamura Motoomi; <sup>c</sup>Nakamura Gakuen, Graduate School of Nutritional Science, Fukuoka, Japan |
| Аффилиация | Takeshita Akira; <sup>a</sup>Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812, Japan |
| Выпуск | 2 |
