| Автор | Forde, B.G. |
| Автор | Heyworth, A. |
| Автор | Pywell, J. |
| Автор | Kreis, M. |
| Дата выпуска | 1985 |
| dc.description | The B-hordeins are the major group of prolamin storage proteins in barley (Hordeum vulgare L.) and they are encoded by a small multigene family that is expressed specifically in the developing endosperm. We report the complete nucleotide sequence of a clone of one B-hordein gene (pBHR184). The cloned gene contains no introns and belongs to the B1 sub-family of B-hordein genes. Comparison of the 5′-flanking sequences of pBHR184 with those of related S-rich prolamin genes from wheat shows that several short sequences within 600 bp upstream of the translation initiation codon are strongly conserved. A sequence that is conserved at around -300 bp in the S-rich prolamins is also conserved at similar locations in genes encoding the two major classes of maize prolamin (the Z19 and Z21 zeins) and appears to be unique to prolamin genes. We discuss the possible role of this ‘-300 element’ in the control of gene expression in the developing cereal endosperm. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © IRL Press Limited |
| Тема | Articles |
| Название | Nucleotide sequence of a B1 hordein gene and the identification of possible upstream regulatory elements in endosperm storage protein genes from barley, wheat and maize |
| Тип | research-article |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 13 |
| Первая страница | 7327 |
| Последняя страница | 7339 |
| Аффилиация | Biochemistry Department, Rothamsted Experimental Station, HarpendenHerts AL5 2JQ, UK |
| Выпуск | 20 |
