| Автор | GHOUSE, A. K. M. |
| Автор | YUNUS, MOHD |
| Дата выпуска | 1974 |
| dc.description | Cuticle-free epidermal peels for the demonstration of primary pit-fields (if any) on the outer surface of the epidermal cells, can be prepared by a chemically controlled process, using 5-10 per cent NaOH solution as a macerating medium. Such peels can be obtained from any desired part of the plant within a period of a week. The process involves allowing the trimmed pieces of the plant material to stand in the NaOH solution at 50–60 °C, until all the cuticular depositions on the outer surface of the epidermal cells are dissolved and the unwanted tissues are sufficiently loosened to allow the easy removal of the epidermis. The separation of epidermis is done by giving moderate agitations in water during washing or by means of a soft-haired brush under a dissecting microscope.The peels so obtained must be washed thoroughly in water and can then be stained with bismark brown or iron alum hematoxylln. After dehydration in ethanol, they can be mounted permanently in Canada balsam. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © Oxford University Press |
| Тема | Articles |
| Название | Preparation of Cuticle-free Epidermal Peels for the Demonstration of Primary Pit-fields |
| Тип | research-article |
| Electronic ISSN | 1095-8290 |
| Print ISSN | 0305-7364 |
| Журнал | Annals of Botany |
| Том | 38 |
| Первая страница | 209 |
| Последняя страница | 211 |
| Аффилиация | Department of Botany, Aligarh Muslim UniversityAligarh, India |
| Выпуск | 1 |
