| Автор | Ruzicka, Jaromir |
| Дата выпуска | 2000 |
| dc.description | This paper introduces a novel methodology for downscaling reagent based assays to micro- and submicroliter level. It is shown that sample handling in the sequential injection mode, which employs forward, reversed and stopped flow, can be programmed to accommodate a wide variety of assays within the same microfluidic device. Solution metering, mixing, dilution, incubation and monitoring can be executed in any desired sequence in a system of channels, integrated with a multipurpose flow cell. The channel system and flow cell are fabricated as a monolithic structure mounted atop a conventional multiposition valve. In addition to compactness, the advantage of this â lab-on-valveâ system is the permanent rigid position of the sample processing channels that ensures repeatability of microfluidic manipulations, controlled by conventional sized peripherals. With the exception of the integrated microconduit system, that has been designed and mesofabricated by computer aided design (CAD) technology, all peripherals (sequential injection system, fiber optic UV/VIS spectrophotometer-fluorometer) are conventional sized and commercially available components. This provides proven robustness and reliability of operation, and makes the microfluidic system compatible with real life samples and peripheral instruments. The system has been characterized by dye injection, to provide guidelines for method development. Its versatility is documented by a phosphate assay, enzymatic activity assay of protease and by a bioligand interaction assay of immunoglobulin G (IgG) based on its interaction with protein G immobilized on Sepharose beads. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | Lab-on-valve: universal microflow analyzer based on sequential and bead injection |
| Тип | ART |
| Журнал | Analyst |
| Том | 125 |
| Первая страница | 1053 |
| Последняя страница | 1060 |
| Аффилиация | Ruzicka Jaromir; Department of Chemistry, University of Washington |
| Выпуск | 6 |
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