Coupling of protein sheet crystals (S-layers) to phospholipid monolayersBasis of a presentation given at Materials Chemistry Discussion No.â 2, 13â 15 September 1999, University of Nottingham, UK.
Weygand, Markus; Schalke, Manfred; HowesCurrent address: University of Leicester, Department of Physics and Astronomy, Leicester, UK LE1 7RH., Paul B.; Kjaer, Kristian; Friedmann, Jaqueline; WetzerCurrent address: Rhône-Poulenc Rorer, F-94403 Vitry-sur-Seine, France., Barbara; Pum, Dietmar; Sleytr, Uwe B.; Lösche, Mathias; Weygand Markus; Leipzig University, Institute of Experimental Physics I; Schalke Manfred; Leipzig University, Institute of Experimental Physics I; HowesCurrent address: University of Leicester, Department of Physics and Astronomy, Leicester, UK LE1 7RH. Paul B.; Risø National Laboratory, Department of Solid State Physics and Chemistry; Kjaer Kristian; Risø National Laboratory, Department of Solid State Physics and Chemistry; Friedmann Jaqueline; University for Agricultural Sciences, Center for Ultrastructure Research and Ludwig-Boltzmann-Institute for Molecular Nanotechnology; WetzerCurrent address: Rhône-Poulenc Rorer, F-94403 Vitry-sur-Seine, France. Barbara; University for Agricultural Sciences, Center for Ultrastructure Research and Ludwig-Boltzmann-Institute for Molecular Nanotechnology; Pum Dietmar; University for Agricultural Sciences, Center for Ultrastructure Research and Ludwig-Boltzmann-Institute for Molecular Nanotechnology; Sleytr Uwe B.; University for Agricultural Sciences, Center for Ultrastructure Research and Ludwig-Boltzmann-Institute for Molecular Nanotechnology; Lösche Mathias; Leipzig University, Institute of Experimental Physics I
Журнал:
Journal of Materials Chemistry
Дата:
2000
Аннотация:
The coupling of bacterial S-layer proteins to phospholipid membranes has been studied in molecular detail with respect to, particularly, the lipid headgroups. Emphasis has been laid on two of the best characterized protein species, the S-layer protein from Bacillus sphaericus CCM2177 and from Bacillus coagulans E38â 66/V1. A combination of fluorescence microscopy, surface sensitive scattering techniques (grazing-incidence X-ray diffraction as well as X-ray and neutron reflectometry) and infrared spectroscopy (FT-IRRAS), applied to surface monolayers of lipids onto which the protein has been reconstituted as continuous molecular crystal sheets, provides a wealth of information which has been utilized to propose detailed molecular models.
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