| Автор | Tabuchi, Mari |
| Автор | Katsuyama, Yoshinori |
| Автор | Nogami, Kazuma |
| Автор | Nagata, Hideya |
| Автор | Wakuda, Keisuke |
| Автор | Fujimoto, Masayuki |
| Автор | Nagasaki, Yukio |
| Автор | Yoshikawa, Kenichi |
| Автор | Kataoka, Kazunori |
| Автор | Baba, Yoshinobu |
| Дата выпуска | 2005 |
| dc.description | We report here advanced microchip electrophoresis using a nanoparticle doped polymer solution that enables greater separation of DNA. The proposed system is simple and effective without any new apparatus or complicated procedures. Various amounts and sizes (80 nm, 110 nm, and 193 nm) of polymer nanoparticle solutions (PEGylated-latex) were mixed with a conventional polymer solution for microchip electrophoresis. When a 0.49 wt% hydroxyl propyl methyl cellulose (HPMC) buffer solution was mixed with a 2.25 wt% 80 nm-PEGylated-latex a higher separation efficiency and a higher mobility of a wider molecular range of dsDNA (10 bp to 2 kbp) was achieved under low viscosity conditions (<5.5 cP) than in conventional 0.7% HPMC. The separation performance was dependent on the particle size and concentration. Furthermore, the effectiveness of the larger PEGylated-latex (193 nm) was not as high as the smaller one (80 to 110 nm). The observed separation improvement by polymer solution with latex-nanoparticles seems to derive from the balance between wider polymer mesh size and the structural obstacles of particles in the buffer. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | A design of nanosized PEGylated-latex mixed polymer solution for microchip electrophoresis |
| Тип | research-article |
| DOI | 10.1039/b410498f |
| Electronic ISSN | 1473-0189 |
| Print ISSN | 1473-0197 |
| Журнал | Lab on a Chip |
| Том | 5 |
| Первая страница | 199 |
| Последняя страница | 204 |
| Аффилиация | Tabuchi Mari; Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, CREST, JST |
| Аффилиация | Katsuyama Yoshinori; Department of Materials Science and Technology, Tokyo University of Science |
| Аффилиация | Nogami Kazuma; Department of Materials Science and Technology, Tokyo University of Science |
| Аффилиация | Nagata Hideya; Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, CREST, JST |
| Аффилиация | Wakuda Keisuke; The Graduate School of Electric Science and Technology and Innovative Joint Research Center, Shizuoka University |
| Аффилиация | Fujimoto Masayuki; The Graduate School of Electric Science and Technology and Innovative Joint Research Center, Shizuoka University |
| Аффилиация | Nagasaki Yukio; Tsukuba Research Center for Interdisciplinary Materials Science, University of Tsukuba |
| Аффилиация | Yoshikawa Kenichi; Department of Physics, Graduate School of Science, Kyoto University |
| Аффилиация | Kataoka Kazunori; Department of Material Science, Graduate School of Engineering, The University of Tokyo, CREST, JST |
| Аффилиация | Baba Yoshinobu; Department of Molecular and Pharmaceutical Biotechnology, Graduate School of Pharmaceutical Sciences, The University of Tokushima, CREST, JST; Department of Applied Chemistry, Graduate School of Engineering, Nagoya University; Single-Molecule Bioanalysis Laboratory, National Institute of Advanced Industrial Science and Technology (AIST) |
| Выпуск | 2 |
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