| Автор | Yang, Xin |
| Автор | Jenkins, Gareth |
| Автор | Franzke, Joachim |
| Автор | Manz, Andreas |
| Дата выпуска | 2005 |
| dc.description | A circular, shear-driven pumping system combined with Fourier Transform detection has been developed for the application of chip based cyclic chromatography. Using this system, it is possible to perform an injection of a sample plug into a circular micro-channel and then drive the sample through the channel, using shear flow induced by a rotation stage. High pumping rates at uniform speeds are attainable with this system with very little heat production. Typical pumping rates of up to 1.423 mm s<sup>â 1</sup> ± 2 µm s<sup>â 1</sup> were used in experiments although much higher rates >14 mm s<sup>â 1</sup> are also possible with this system. Fluorescence detection was used to detect a sample plug of Coumarin dyes, flowing around the channel. A coating of porous polymethacrylate was used to immobilise RP-HPLC beads onto the glass surface and was applied to the glass micro-channel by selective photopolymerisation. This coating acted as a stationary phase and differences in retention time were observed for an injection of Coumarin dyes for different methanolâ water, mobile phase ratios. Full sample retention occurred for 30 : 70 (v/v) methanolâ water whereas no retention occurred for 92 : 8 (v/v) methanolâ water which can be expected for such a reverse phase, open tubular system. Fourier transform detection applied to the fully retained and non-retained cases showed frequency domain data from a single detection point corresponding to that which may be expected from shear flow theory. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | Shear-driven pumping and Fourier transform detection for on chip circular chromatography applications |
| Тип | research-article |
| DOI | 10.1039/b502121a |
| Electronic ISSN | 1473-0189 |
| Print ISSN | 1473-0197 |
| Журнал | Lab on a Chip |
| Том | 5 |
| Первая страница | 764 |
| Последняя страница | 771 |
| Аффилиация | Yang Xin; Imperial College London, Department of Chemistry |
| Аффилиация | Jenkins Gareth; ISASâ Institute for Analytical Sciences |
| Аффилиация | Franzke Joachim; ISASâ Institute for Analytical Sciences |
| Аффилиация | Manz Andreas; ISASâ Institute for Analytical Sciences |
| Выпуск | 7 |
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