| Автор | Koskinen, Janne O. |
| Автор | Meltola, Niko J. |
| Автор | Soini, Erkki |
| Автор | Soini, Aleksi E. |
| Дата выпуска | 2005 |
| dc.description | A new lab-on-a-chip compatible binding assay platform is introduced. The platform combines dry-chemistry bioaffinity reagents and the recently introduced ArcDia TPX<sup>⠢</sup> binding assay technique. The technique employs polymer microspheres as a solid phase reaction carrier, fluorescently labeled antibody conjugates, and detection of fluorescence emission from the surface of individual microspheres by two-photon excitation fluorescence. Signal response of the technique is independent of the reaction volume, thus the technique is particularly well suited for detection of bioaffinity reactions from miniature volumes. Performance of the new assay platform is studied by means of an immunometric assay of human α-fetoprotein (hAFP) in 384-plate format, and the results are compared to those of a corresponding wet-chemistry assay method. The results show that the ArcDia TPX<sup>⠢</sup> detection technique can be combined with dry-chemistry reagents without compromises in assay performance. The microchip field has so far been characterized with a lack of microchip-compatible detection platforms which would allow cost-effective microchip design and sensitive bioaffinity detection. The presented detection technique is expected to provide a solution for this shortage. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | A lab-on-a-chip compatible bioaffinity assay method for human α-fetoprotein |
| Тип | research-article |
| DOI | 10.1039/b509333c |
| Electronic ISSN | 1473-0189 |
| Print ISSN | 1473-0197 |
| Журнал | Lab on a Chip |
| Том | 5 |
| Первая страница | 1408 |
| Последняя страница | 1411 |
| Аффилиация | Koskinen Janne O.; Laboratory of Biophysics, Institute of Biomedicine, University of Turku |
| Аффилиация | Meltola Niko J.; Laboratory of Biophysics, Institute of Biomedicine, University of Turku |
| Аффилиация | Soini Erkki; Arctic Diagnostics Oy |
| Аффилиация | Soini Aleksi E.; Arctic Diagnostics Oy |
| Выпуск | 12 |
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