A new approach for DNA detection by SERRS
Faulds, Karen; Fruk, Ljiljana; Robson, David C.; Thompson, David G.; Enright, Alexis; Ewen Smith, W.; Graham, Duncan; Faulds Karen; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Fruk Ljiljana; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Robson David C.; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Thompson David G.; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Enright Alexis; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Ewen Smith W.; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde; Graham Duncan; Duncan Graham, Department of Pure and Applied Chemistry, University of Strathclyde
Журнал:
Faraday Discussions
Дата:
2006
Аннотация:
A new approach for the detection of DNA using surface enhance resonance Raman scattering (SERRS) is reported. The majority of existing techniques use fluorescence spectroscopy with advanced probe design to provide information on the identity of specific DNA sequences down to single base resolution. A new approach to the labelling of DNA is discussed which uses Michael addition to couple thiolated DNA to dye labels specifically designed to attach to silver surfaces. When combined with existing strategies for sensitive detection of DNA using commercially available labels, a new class of biomolecular probe known as a SERRS Beacon was produced. The detection techniques of fluorescence and surface enhanced resonance Raman scattering (SERRS) are combined to give a sensitive and selective system for use in the development and creation of novel assays for specifically defined targets. It demonstrates improved potential for multiplexing analysis.
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