A bio-barcode assay for on-chip attomolar-sensitivity protein detection
Goluch, Edgar D.; Nam, Jwa-Min; Georganopoulou, Dimitra G.; Chiesl, Thomas N.; Shaikh, Kashan A.; Ryu, Kee S.; Barron, Annelise E.; Mirkin, Chad A.; Liu, Chang; Goluch Edgar D.; Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign; NamCurrent address: Department of Chemistry, Seoul National University, Seoul, 151-747, South Korea. Jwa-Min; Department of Chemistry and Institute of Nanotechnology, Northwestern University; Georganopoulou Dimitra G.; Department of Chemistry and Institute of Nanotechnology, Northwestern University; Chiesl Thomas N.; Department of Chemical Engineering, Northwestern University; Shaikh Kashan A.; Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign; Ryu Kee S.; Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign; Barron Annelise E.; Department of Chemical Engineering, Northwestern University; Mirkin Chad A.; Department of Chemistry and Institute of Nanotechnology, Northwestern University; Liu Chang; Micro and Nanotechnology Laboratory, Bioengineering Department, University of Illinois at Urbana-Champaign
Журнал:
Lab on a Chip
Дата:
2006
Аннотация:
Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticlesâ a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 µL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.
523.6Кб