Chapter 7. Capturing a Single Cell
Rey, Catherine; Wierinckx, Anne; Croze, Séverine; Legras-Lachuer, Catherine; Lachuer, Joel; Rey Catherine; Genomic and microgenomic core facility: ProfileXpert platform (Federative Institute of Neurosciences); Institut Fédératif des Neurosciences; Wierinckx Anne; Genomic and microgenomic core facility: ProfileXpert platform (Federative Institute of Neurosciences); Université de Lyon 1; Croze Séverine; Genomic and microgenomic core facility: ProfileXpert platform (Federative Institute of Neurosciences); Legras-Lachuer Catherine; Genomic and microgenomic core facility: ProfileXpert platform (Federative Institute of Neurosciences); Université de Lyon, INRA, UMR754, Université Lyon 1; Lachuer Joel; Genomic and microgenomic core facility: ProfileXpert platform (Federative Institute of Neurosciences); Université de Lyon 1
Журнал:
Unravelling Single Cell Genomics
Дата:
2010
Аннотация:
A major problem encountered in genomic and proteomic studies arises from the heterogeneous nature of different tissue. Analysis of a pure cell population is essential for correlating relevant molecular signatures in diseased and disease-free cells. During the last 30 years this challenge has led to the development of different technologies able to isolate cells of interest. Laser capture microdissection (LCM) is the last available technology using the precision of a laser beam to isolate single cells from complex tissue. In this chapter we will review the different technologies available and some applications.
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