| Автор | Walker, Glenn M. |
| Автор | Monteiro-Riviere, Nancy |
| Автор | Rouse, Jillian |
| Автор | O'Neill, Adrian T. |
| Дата выпуска | 2007 |
| dc.description | A two-layer polymer microfluidic device is presented which creates nine linear dilutions from two input fluid streams mixed in varying volumetric proportions. The linearity of the nine dilutions is conserved when the flow rate is held constant at 1.0 µl min<sup>â 1</sup> (R<sup>2</sup> = 0.9995) and when it is varied from 0.5â 16 µl min<sup>â 1</sup> (R<sup>2</sup> = 0.9998). An analytical expression is presented for designing microfluidic devices with arbitrary numbers of linear dilutions. To demonstrate the efficacy of this device, primary human epidermal keratinocytes (HEK) were stained with nine dilutions of calcein, resulting in a linear spread of fluorescent intensities (R<sup>2</sup> = 0.94). The operating principles of the device can be scaled up to incorporate any number of linear dilutions. This scalability, coupled with an intrinsic ability to create linear dilutions under a variety of operating conditions, makes the device applicable to high throughput screening applications such as combinatorial chemistry or cytotoxicity assays. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | A linear dilution microfluidic device for cytotoxicity assays |
| Тип | research-article |
| DOI | 10.1039/b608990a |
| Electronic ISSN | 1473-0189 |
| Print ISSN | 1473-0197 |
| Журнал | Lab on a Chip |
| Том | 7 |
| Первая страница | 226 |
| Последняя страница | 232 |
| Аффилиация | Walker Glenn M.; Joint Department of Biomedical Engineering at The University of North Carolina at Chapel Hill and North Carolina State University |
| Аффилиация | Monteiro-Riviere Nancy; The Center for Chemical Toxicology Research and Pharmacokinetics within the College of Veterinary Medicine at North Carolina State University |
| Аффилиация | Rouse Jillian; Joint Department of Biomedical Engineering at The University of North Carolina at Chapel Hill and North Carolina State University; The Center for Chemical Toxicology Research and Pharmacokinetics within the College of Veterinary Medicine at North Carolina State University |
| Аффилиация | O'Neill Adrian T.; Joint Department of Biomedical Engineering at The University of North Carolina at Chapel Hill and North Carolina State University |
| Выпуск | 2 |
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