| Автор | Cheng, Shing-Yi |
| Автор | Heilman, Steven |
| Автор | Wasserman, Max |
| Автор | Archer, Shivaun |
| Автор | Shuler, Michael L. |
| Автор | Wu, Mingming |
| Дата выпуска | 2007 |
| dc.description | We have developed a hydrogel-based microfluidic device that is capable of generating a steady and long term linear chemical concentration gradient with no through flow in a microfluidic channel. Using this device, we successfully monitored the chemotactic responses of wildtype Escherichia coli (suspension cells) to α-methyl-dl-aspartate (attractant) and differentiated HL-60 cells (a human neutrophil-like cell line that is adherent) to formyl-Met-Leu-Phe (f-MLP, attractant). This device advances the current state of the art in microchemotaxis devices in that (1) it demonstrates the validity of using hydrogels as the building material for a microchemotaxis device; (2) it demonstrates the potential of the hydrogel based microfluidic device in biological experiments since most of the proteins and nutrients essential for cell survival are readily diffusible in hydrogel; (3) it is capable of applying chemical stimuli independently of mechanical stimuli; (4) it is straightforward to make, and requires very basic tools that are commonly available in biological labs. This device will also be useful in controlling the chemical and mechanical environment during the formation of tissue engineered constructs. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | A hydrogel-based microfluidic device for the studies of directed cell migration |
| Тип | research-article |
| DOI | 10.1039/b618463d |
| Electronic ISSN | 1473-0189 |
| Print ISSN | 1473-0197 |
| Журнал | Lab on a Chip |
| Том | 7 |
| Первая страница | 763 |
| Последняя страница | 769 |
| Аффилиация | Cheng Shing-Yi; School of Chemical and Biomolecular Engineering, Cornell University |
| Аффилиация | Heilman Steven; School of Chemical and Biomolecular Engineering, Cornell University |
| Аффилиация | Wasserman Max; Sibley School of Mechanical and Aerospace Engineering, Cornell University |
| Аффилиация | Archer Shivaun; Department of Biomedical Engineering, Cornell University |
| Аффилиация | Shuler Michael L.; School of Chemical and Biomolecular Engineering, Cornell University; Department of Biomedical Engineering, Cornell University |
| Аффилиация | Wu Mingming; School of Chemical and Biomolecular Engineering, Cornell University; Sibley School of Mechanical and Aerospace Engineering, Cornell University |
| Выпуск | 6 |
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