A chip-based platform for the in vitro generation of tissues in three-dimensional organization
Gottwald, Eric; Giselbrecht, Stefan; Augspurger, Caroline; Lahni, Brigitte; Dambrowsky, Nina; Truckenmüller, Roman; Piotter, Volker; Gietzelt, Thomas; Wendt, Oliver; Pfleging, Wilhelm; Welle, Alex; Rolletschek, Alexandra; Wobus, Anna M.; Weibezahn, Karl-Friedrich; Gottwald Eric; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Giselbrecht Stefan; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Augspurger Caroline; Center of Innovation Competence MacroNano<sup>®</sup>, Institute for Micro- and Nanotechnologies, Technische Universität Ilmenau; Lahni Brigitte; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Dambrowsky Nina; Atotech Deutschland GmbH; Truckenmüller Roman; Institute of Microstructure Technology, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Piotter Volker; Institute for Materials Research III, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Gietzelt Thomas; Institute for Micro Process Engineering, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Wendt Oliver; Draeger Medical AG & Co. KG, Moislinger Allee 53-55; Pfleging Wilhelm; Institute for Materials Research I, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Welle Alex; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Rolletschek Alexandra; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1; Leibniz Institute for Plant Genetics and Crop Plant Research, In Vitro Differentiation Group; Wobus Anna M.; Leibniz Institute for Plant Genetics and Crop Plant Research, In Vitro Differentiation Group; Weibezahn Karl-Friedrich; Institute for Biological Interfaces, Forschungszentrum Karlsruhe, Hermann-von-Helmholtz-Platz 1
Журнал:
Lab on a Chip
Дата:
2007
Аннотация:
We describe a multi-purpose platform for the three-dimensional cultivation of tissues. The device is composed of polymer chips featuring a microstructured area of 1â 2 cm<sup>2</sup>. The chip is constructed either as a grid of micro-containers measuring 120â 300 à 300 à 300 µm (h à l à w), or as an array of round recesses (300 µm diameter, 300 µm deep). The micro-containers may be separately equipped with addressable 3D-micro-electrodes, which allow for electrical stimulation of excitable cells and on-site measurements of electrochemically accessible parameters. The system is applicable for the cultivation of high cell densities of up to 8 à 10<sup>6</sup> cells and, because of the rectangular grid layout, allows the automated microscopical analysis of cultivated cells. More than 1000 micro-containers enable the parallel analysis of different parameters under superfusion/perfusion conditions. Using different polymer chips in combination with various types of bioreactors we demonstrated the principal suitability of the chip-based bioreactor for tissue culture applications. Primary and established cell lines have been successfully cultivated and analysed for functional properties. When cells were cultured in non-perfused chips, over time a considerable degree of apoptosis could be observed indicating the need for an active perfusion. The system presented here has also been applied for the differentiation analysis of pluripotent embryonic stem cells and may be suitable for the analysis of the stem cell niche.
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