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Автор Sun, Y.
Автор Kwok, Y. C.
Автор Nguyen, N. T.
Дата выпуска 2007
dc.description In the past few years, much attention has been paid to the development of miniaturized polymerase chain reaction (PCR) devices. After a continuous flow (CF) PCR chip was introduced, several CFPCR systems employing various pumping mechanisms were reported. However, the use of pumps increases cost and imposes a high requirement on microchip bonding integrity due to the application of high pressure. Other significant limitations of CFPCR devices include the large footprint of the microchip and the fixed cycle number which is dictated by the channel layout. In this paper, we present a novel circular close-loop ferrofluid driven microchip for rapid PCR. A small ferrofluid plug, containing sub-domain magnetic particles in a liquid carrier, is driven by an external magnet along the circular microchannel, which in turn propels the PCR mixture through three temperature zones. Amplification of a 500 bp lambda DNA fragment has been demonstrated on the polymethyl methacrylate (PMMA) PCR microchip fabricated by CO2 laser ablation and bonded by a low pressure, high temperature technique. Successful PCR was achieved in less than 4 min. Effects of cycle number and cycle time on PCR products were investigated. Using a magnet as the actuator eliminates the need for expensive pumps and provides advantages of low cost, small power consumption, low requirement on bonding strength and flexible number of PCR cycles. Furthermore, the microchip has a much simpler design and smaller footprint compared to the rectangular serpentine CFPCR devices. To demonstrate its application in forensics, a 16-loci short tandem repeat (STR) sample was successfully amplified using the PCR microchip.
Формат application.pdf
Издатель Royal Society of Chemistry
Название A circular ferrofluid driven microchip for rapid polymerase chain reaction
Тип research-article
DOI 10.1039/b700575j
Electronic ISSN 1473-0189
Print ISSN 1473-0197
Журнал Lab on a Chip
Том 7
Первая страница 1012
Последняя страница 1017
Аффилиация Sun Y.; National Institute of Education, Nanyang Technological University
Аффилиация Kwok Y. C.; National Institute of Education, Nanyang Technological University
Аффилиация Nguyen N. T.; School of Mechanical and Aerospace Engineering, Nanyang Technological University
Выпуск 8
Библиографическая ссылка Woolley, Anal. Chem., 1996, 68, 4081
Библиографическая ссылка Sun, Anal. Chim. Acta, 2006, 556, 80
Библиографическая ссылка Oda, Anal. Chem., 1998, 70, 918
Библиографическая ссылка Roper, Anal. Chem., 2005, 77, 3887
Библиографическая ссылка Lee, Lab Chip, 2004, 4, 401
Библиографическая ссылка Kopp, Science, 1998, 280, 1046
Библиографическая ссылка Obeid, Anal. Chem., 2003, 75, 288
Библиографическая ссылка Curcio, Anal. Chem., 2003, 75, 1
Библиографическая ссылка Belgrader, Anal. Chem., 2003, 75, 3446
Библиографическая ссылка Chou, Microelectron. Eng., 2002, 61, 921
Библиографическая ссылка Chou, Biomed. Microdev., 2001, 3, 323
Библиографическая ссылка Krishnan, Science, 2002, 298, 793
Библиографическая ссылка Krishnan, Anal. Chem., 2004, 76, 6254
Библиографическая ссылка Chen, Anal. Chem., 2004, 76, 3707
Библиографическая ссылка Wheeler, Anal. Chem., 2004, 76, 4011
Библиографическая ссылка Hatch, J. Microelectromech. Syst., 2001, 10, 215
Библиографическая ссылка Yamahata, J. Microelectromech. Syst., 2005, 14, 96
Библиографическая ссылка Nguyen, Appl. Phys. Lett, 2006, 89, 052509-1
Библиографическая ссылка Sun, J. Micromech. Microeng., 2006, 16, 1681
Библиографическая ссылка Hu, Anal. Chim. Acta, 2006, 557, 146
Библиографическая ссылка Kim, Biochem. Eng. J., 2006, 29, 91

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