| Автор | Yuan, Haodan |
| Автор | Mullett, Wayne M. |
| Автор | Pawliszyn, Janusz |
| Дата выпуска | 2001 |
| dc.description | A theophylline antiserum was covalently immobilized on the surface of a fused silica fiber, modified with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde, and used as a selective and sensitive extraction medium for the immunoaffinity solid-phase microextraction (SPME) determination of theophylline in serum samples. The specificity of the immunoaffinity SPME fiber was first investigated using a fixed concentration of [<sup>3</sup>H]theophylline together with various amounts of interference, possessing no cross-reactivity with the theophylline antibody. No significant non-specific binding was observed. The reproducibility of the fiber preparation and the immunoaffinity SPME analysis was also investigated, resulting in a relative standard deviation of 6.1% for five analyses of the same fiber. The antigenâ antibody binding isotherm was obtained by analyzing theophylline standards of various concentrations (0.1â 5 ng mL<sup>â 1</sup>) until saturation values were reached. Initial binding of theophylline was linear with a r<sup>2</sup> = 0.968. The cross-reactivity of the theophylline immunoaffinity SPME fiber for the structural analog caffeine was investigated by adding various amounts of caffeine in the presence of theophylline at a saturation concentration and produced a low cross-reactivity value of 0.1%. Finally, spiked serum samples (10 and 50 ng mL<sup>â 1</sup>) were successfully analyzed with an excellent correlation with the standard binding isotherm, thus confirming the performance of the immunoaffinity SPME coating for improved bioanalysis. |
| Формат | application.pdf |
| Издатель | Royal Society of Chemistry |
| Название | Biological sample analysis with immunoaffinity solid-phase microextraction |
| Тип | research-article |
| DOI | 10.1039/b101854j |
| Electronic ISSN | 1364-5528 |
| Print ISSN | 0003-2654 |
| Журнал | Analyst |
| Том | 126 |
| Первая страница | 1456 |
| Последняя страница | 1461 |
| Аффилиация | Yuan Haodan; Department of Chemistry, University of Waterloo |
| Аффилиация | Mullett Wayne M.; Department of Chemistry, University of Waterloo |
| Аффилиация | Pawliszyn Janusz; Department of Chemistry, University of Waterloo |
| Выпуск | 8 |
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