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Автор Gallardo-Moreno, Amparo M.
Автор Luisa González-Martín, M.
Автор Bruque, José M.
Автор Pérez-Giraldo, Ciro
Дата выпуска 2004
dc.description The initial microbial adhesion of bacteria to different surfaces seems to be mediated by physico-chemical forces and this is the reason why the physico-chemical surface characterisation of bacteria has recently gained interest. In this context, the adhesion of different microorganisms to biological substrata has been described from a physico-chemical point of view, aiming to simulate, as closely as possible, the conditions of interest. On this basis, the objective of this work is to characterise the surface of Enterococcus faecalis ATCC29212 through hydrophobicity, surface free energy and zeta potential at 37â °C, when cells grow in Trypticase Soy Broth (TSB) and TSB supplemented with serum or urine. These variations are used to provide a theoretical description of the bacterial adhesion to glass by interaction free energy, which is verified with experimental results employing a parallel plate flow chamber set at 37â °C, to simulate the conditions of flow inside the human body. The results show that the addition of serum to the growth medium increases the hydrophobicity and isoelectric point (i.e.p.) of the microorganisms, and this could indicate an increase in the protein content on the cell surface. However urine does not introduce a change in the above magnitudes. At short separation distances between the cells and the substratum, the interaction free energy predicts a favourable adhesion for serum-grown cells while non-favourable adhesion is expected for control (TSB-grown cells) and urine-grown cells. These results are in agreement with the experimental adhesion data, obtained with the flow chamber.
Формат application.pdf
Издатель Royal Society of Chemistry
Название Changes on the physico-chemical surface properties and adhesion behaviour of Enterococcus faecalis by the addition of serum or urine to the growth medium
Тип research-article
DOI 10.1039/b314609j
Electronic ISSN 1463-9084
Print ISSN 1463-9076
Журнал Physical Chemistry Chemical Physics
Том 6
Первая страница 1512
Последняя страница 1517
Аффилиация Gallardo-Moreno Amparo M.; Department of Physics, University of Extremadura
Аффилиация Luisa González-Martín M.; Department of Physics, University of Extremadura
Аффилиация Bruque José M.; Department of Physics, University of Extremadura
Аффилиация Pérez-Giraldo Ciro; Department of Microbiology, University of Extremadura
Выпуск 7
Библиографическая ссылка Piette, Biofouling, 1991, 5, 3
Библиографическая ссылка Cooksey, Aquat. Microb. Ecol., 1995, 9, 87
Библиографическая ссылка Addy, J. Appl. Bacteriol., 1992, 73, 269
Библиографическая ссылка Sartingen, Infect. Immun., 2000, 68, 6044
Библиографическая ссылка Landman, J. Antimicrob. Chemother., 1997, 40, 161
Библиографическая ссылка Handbook of bacterial adhesion. Principles, methods and applications, ed. Y. H. An and R. J. Friedman, Humana Press Inc., Totowa, NJ, 2000
Библиографическая ссылка Tunney, Int. J. Pharm., 1997, 151, 121
Библиографическая ссылка Tieszer, J. Urol., 1998, 160, 876
Библиографическая ссылка Van Loosdrecht, Aquat. Sci., 1990, 52, 103
Библиографическая ссылка Bos, FEMS Microbiol. Rev., 1999, 23, 179
Библиографическая ссылка Gallardo-Moreno, Colloids Surf. B, 2002, 24, 285
Библиографическая ссылка Azeredo, Colloids Surf. B, 1999, 14, 141
Библиографическая ссылка van Oss, J. Colloid Interface Sci., 1986, 111, 378
Библиографическая ссылка van Oss, Langmuir, 1988, 4, 884
Библиографическая ссылка Sjollema, J. Microbiol. Meth., 1989, 9, 73
Библиографическая ссылка Busscher, Appl. Environ. Microbiol., 1984, 48, 980
Библиографическая ссылка González-Martín, Langmuir, 1997, 13, 5991
Библиографическая ссылка Gallardo-Moreno, Colloids Surf. B, 2003, 28, 119
Библиографическая ссылка van Oss, J. Adhes., 1997, 63, 71
Библиографическая ссылка Gallardo-Moreno, J. Adhes. Sci. Technol., 2003, 17, 1877
Библиографическая ссылка van der Mei, Microbiology, 1994, 140, 3413
Библиографическая ссылка Lytle, Colloids Surf. B, 2002, 24, 91
Библиографическая ссылка van der Mei, Adv. Dent. Res., 1997, 11, 388
Библиографическая ссылка van der Mei, Archs. Oral Biol., 1989, 34, 889
Библиографическая ссылка Ljungh, Microbiol. Immunol., 1995, 39, 753
Библиографическая ссылка Lyklema, Colloids Surf., 1989, 39, 175
Библиографическая ссылка van Oss, J. Mol. Recognit., 1990, 3, 128

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