| Автор | Carey, Paul R. |
| Дата выпуска | 2006 |
| dc.description | ▪ Abstract Recent studies using a Raman microscope have shown that single protein crystals provide an ideal platform to undertake Raman difference spectroscopic analyses under nonresonance conditions. This approach, termed Raman crystallography, provides a means of characterizing chemical events within the crystal such as ligand binding and enzyme reactions. In many cases Raman crystallography goes hand in hand with X-ray crystallographic studies because the Raman results can inform the X-ray crystallographer about the status of chemical events in the crystal prior to flash freezing and X-ray analysis. In turn, the combined data from the Raman and X-ray analyses are highly synergistic and offer novel perspectives on structure and dynamics in enzyme active sites. In a related area, protein misfolding, Raman microscopy can provide detailed insights into the chemistry of the amyloid plaques associated with Alzheimer's disease and into the intermediates on the α-synuclein protein misfolding pathway implicated in Parkinson's disease. |
| Формат | application.pdf |
| Издатель | Annual Reviews |
| Копирайт | Annual Reviews |
| Название | RAMAN CRYSTALLOGRAPHY AND OTHER BIOCHEMICAL APPLICATIONS OF RAMAN MICROSCOPY |
| DOI | 10.1146/annurev.physchem.57.032905.104521 |
| Print ISSN | 0066-426X |
| Журнал | Annual Review of Physical Chemistry |
| Том | 57 |
| Первая страница | 527 |
| Последняя страница | 554 |
| Аффилиация | Carey, Paul R.; Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106; email: paul.carey@case.edu |
