| Автор | Chan, Po-Hsiung |
| Автор | Su, Yu-Chuan |
| Дата выпуска | 2007-03-01 |
| dc.description | This work presents a micro osmotic screening system that grows protein crystals in hours while consuming only micrograms of samples. Throughout the crystallization process, water can be driven in or out of a protein solution (across a semi-permeable membrane) to adjust its concentrations as desired. With the bi-directional and adjustable flow control realized by osmosis, each protein sample can be screened for crystallization conditions over a highly extended range. In the prototype demonstration, 6 × 8 screening arrays having an overall size of 20 × 24 × 2.5 mm<sup>3</sup> were fabricated and characterized with crystallization experiments. In these experiments, crystallization conditions for four proteins, including lysozyme, catalase, thaumatin and xylanase, were identified within 2–6 h while consuming less than 20 µl of sample solution for each protein. Furthermore, it was also demonstrated that diffraction-quality crystals may be grown and harvested from the prototype system. As such, this osmotic system pioneers a new class of rapid screening schemes for high-throughput protein crystallization. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2007 IOP Publishing Ltd |
| Название | Rapid protein crystallization by a micro osmotic screening systemA portion of this paper was presented at the 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Tokyo, Japan, November 2006. |
| Тип | paper |
| DOI | 10.1088/0960-1317/17/3/029 |
| Electronic ISSN | 1361-6439 |
| Print ISSN | 0960-1317 |
| Журнал | Journal of Micromechanics and Microengineering |
| Том | 17 |
| Первая страница | 642 |
| Последняя страница | 650 |
| Последняя страница | 650 |
| Аффилиация | Chan, Po-Hsiung; Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan |
| Аффилиация | Su, Yu-Chuan; Department of Engineering and System Science, National Tsing Hua University, Hsinchu, Taiwan |
| Выпуск | 3 |
