We study the effect of brane fluctuation on the indirect signals of high energy colliders. Brane fluctuation could act as a regulator of divergent expression of infinite tower of Kaluza-Klein graviton effects. The phenomenological parameter λ, introduced by Hewett, is shown to be determined in our setting, and its dramatic behaviors depending on the D = 4+δ dimensional gravitation scale M_D, `softening parameter' Δ, and (s)^1/2 of collider are presented. The present exclusion bounds from the processes e⁺e⁻→γγ and pp̅→e⁺e⁻,γγ(γ) are considered within the parameter space (M_D,Δ) with respect to the number of extra dimensions.