| Автор | Gladilin, E |
| Автор | Micoulet, A |
| Автор | Hosseini, B |
| Автор | Rohr, K |
| Автор | Spatz, J |
| Автор | Eils, R |
| Дата выпуска | 2007-06-01 |
| dc.description | Mechanical forces play an important role in many microbiological phenomena such as embryogenesis, regeneration, cell proliferation and differentiation. Micromanipulation of cells in a controlled environment is a widely used approach for understanding cellular responses with respect to external mechanical forces. While modern micromanipulation and imaging techniques provide useful optical information about the change of overall cell contours under the impact of external loads, the intrinsic mechanisms of energy and signal propagation throughout the cell structure are usually not accessible by direct observation. This work deals with the computational modelling and simulation of intracellular strain state of uniaxially stretched cells captured in a series of images. A nonlinear elastic finite element method on tetrahedral grids was applied for numerical analysis of inhomogeneous stretching of a rat embryonic fibroblast 52 (REF 52) using a simplified two-component model of a eukaryotic cell consisting of a stiffer nucleus surrounded by a softer cytoplasm. The difference between simulated and experimentally observed cell contours is used as a feedback criterion for iterative estimation of canonical material parameters of the two-component model such as stiffness and compressibility. Analysis of comparative simulations with varying material parameters shows that (i) the ratio between the stiffness of cell nucleus and cytoplasm determines intracellular strain distribution and (ii) large deformations result in increased stiffness and decreased compressibility of the cell cytoplasm. The proposed model is able to reproduce the evolution of the cellular shape over a sequence of observed deformations and provides complementary information for a better understanding of mechanical cell response. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2007 IOP Publishing Ltd |
| Название | 3D finite element analysis of uniaxial cell stretching: from image to insight |
| Тип | paper |
| DOI | 10.1088/1478-3975/4/2/004 |
| Electronic ISSN | 1478-3975 |
| Print ISSN | 1478-3967 |
| Журнал | Physical Biology |
| Том | 4 |
| Первая страница | 104 |
| Последняя страница | 113 |
| Последняя страница | 113 |
| Аффилиация | Gladilin, E; German Cancer Research Centre, Department Theoretical Bioinformatics, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany |
| Аффилиация | Micoulet, A; >Department Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, INF 253, D-69120 Heidelberg, Germany |
| Аффилиация | Hosseini, B; >Department Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, INF 253, D-69120 Heidelberg, Germany |
| Аффилиация | Rohr, K; German Cancer Research Centre, Department Theoretical Bioinformatics, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany; Institute for Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany |
| Аффилиация | Spatz, J; >Department Biophysical Chemistry, Institute for Physical Chemistry, University of Heidelberg, INF 253, D-69120 Heidelberg, Germany; Max-Planck-Institute for Metals Research, Department New Materials and Biosystems, Heisenbergstrasse 3, D-70569 Stuttgart, Germany |
| Аффилиация | Eils, R; German Cancer Research Centre, Department Theoretical Bioinformatics, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany; Institute for Pharmacy and Molecular Biotechnology, University of Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany |
| Выпуск | 2 |
