| Автор | Werner, Maria |
| Автор | Aurell, Erik |
| Дата выпуска | 2009-12-01 |
| dc.description | We present a comprehensive, computational study of the properties of bacteriophage λ mutants designed by Atsumi and Little (2006 Proc. Natl. Acad. Sci. 103 4558-63). These phages underwent a genetic reconstruction where Cro was replaced by a dimeric form of the Lac repressor. To clarify the theoretical characteristics of these mutants, we built a detailed thermodynamic model. The mutants all have a different genetic wiring than the wild-type λ. One group lacks regulation of P<sub>RM</sub> by the lytic protein. These mutants only exhibit the lysogenic equilibrium, with no transiently active P<sub>R</sub>. The other group lacks the negative feedback from CI. In this group, we identify a handful of bi-stable mutants, although the majority only exhibit the lysogenic equilibrium. The experimental identification of functional phages differs from our predictions. From a theoretical perspective, there is no reason why only 4 out of 900 mutants should be functional. The differences between theory and experiment can be explained in two ways. Either, the view of the λ phage as a bi-stable system needs to be revised, or the mutants have in fact not undergone a modular replacement, as intended by Atsumi and Little, but constitute instead a wider systemic change. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2009 IOP Publishing Ltd |
| Название | A computational study of λ-lac mutants |
| Тип | paper |
| DOI | 10.1088/1478-3975/6/4/046007 |
| Electronic ISSN | 1478-3975 |
| Print ISSN | 1478-3967 |
| Журнал | Physical Biology |
| Том | 6 |
| Первая страница | 46007 |
| Последняя страница | 46017 |
| Аффилиация | Werner, Maria; Department of Computational Biology, KTH—Royal Institute of Technology, Albanova University Center, SE-10691 Stockholm, Sweden |
| Аффилиация | Aurell, Erik; Department of Computational Biology, KTH—Royal Institute of Technology, Albanova University Center, SE-10691 Stockholm, Sweden |
| Выпуск | 4 |
