| Автор | Liao, Susan |
| Автор | Ngiam, Michelle |
| Автор | Chan, Casey K |
| Автор | Ramakrishna, S |
| Дата выпуска | 2009-04-01 |
| dc.description | Mineralized type I collagen (collagen I) nanofibers and their nanofibril bundles make up the microstructure of natural bone tissue, which range from nanometers to micrometers. However, attempts to achieve this hierarchically assembled structure in vitro have been unsuccessful. In this study, we added osteonectin into the collagen I solution, either at a high or low weight ratio (osteonectin: collagen I = 1:30 or 1:90) before co-precipitation. Results indicated that spindle-like nano-hydroxyapatite (nano-HA) was deposited on collagen/osteonectin and pure osteonectin (control) groups. Furthermore, transmission electron microscope (TEM) and scanning electron microscope (SEM) results showed that the assembled mineralized fiber bundles were formed randomly at different levels from 50 nm, 250 nm to 1100 nm. However, when we replaced collagen I with collagen II, osteonectin addition did not induce the formation of mineralized fiber bundles. The participation of osteonectin in the assembly of the mineralized fibers could provide new insights into the novel mineralization function of osteonectin for bone development in vivo and advancing new biomimetic methods for bone graft applications. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2009 IOP Publishing Ltd |
| Название | Fabrication of nano-hydroxyapatite/collagen/osteonectin composites for bone graft applicationsThis work was presented at the 2008 Symposium on Biomaterials and Bionanotechnology (SBB), part of the International Conference on Multifunctional Materials and Structures (MFMS2008), Hong Kong, 28–31 July 2008. |
| Тип | paper |
| DOI | 10.1088/1748-6041/4/2/025019 |
| Electronic ISSN | 1748-605X |
| Print ISSN | 1748-6041 |
| Журнал | Biomedical Materials |
| Том | 4 |
| Первая страница | 25019 |
| Последняя страница | 25026 |
| Аффилиация | Liao, Susan; Division of Bioengineering, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 119074, Singapore; |
| Аффилиация | Ngiam, Michelle; Division of Bioengineering, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Graduate Programme in Bioengineering, Graduate School for Integrative Sciences and Engineering, Centre for Life Sciences, National University of Singapore, 117456, Singapore |
| Аффилиация | Chan, Casey K; Division of Bioengineering, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 119074, Singapore |
| Аффилиация | Ramakrishna, S; Division of Bioengineering, Nanoscience and Nanotechnology Initiative, Faculty of Engineering, National University of Singapore, 117576, Singapore; Department of Mechanical Engineering, Faculty of Engineering, National University of Singapore, 117576, Singapore |
| Выпуск | 2 |
