| Автор | Xu, F |
| Автор | Moon, S J |
| Автор | Emre, A E |
| Автор | Turali, E S |
| Автор | Song, Y S |
| Автор | Hacking, S A |
| Автор | Nagatomi, J |
| Автор | Demirci, U |
| Дата выпуска | 2010-03-01 |
| dc.description | Tissue engineering based on building blocks is an emerging method to fabricate 3D tissue constructs. This method requires depositing and assembling building blocks (cell-laden microgels) at high throughput. The current technologies (e.g., molding and photolithography) to fabricate microgels have throughput challenges and provide limited control over building block properties (e.g., cell density). The cell-encapsulating droplet generation technique has potential to address these challenges. In this study, we monitored individual building blocks for viability, proliferation and cell density. The results showed that (i) SMCs can be encapsulated in collagen droplets with high viability (>94.2 ± 3.2%) for four cases of initial number of cells per building block (i.e. 7 ± 2, 16 ± 2, 26 ± 3 and 37 ± 3 cells/building block). (ii) Encapsulated SMCs can proliferate in building blocks at rates that are consistent (1.49 ± 0.29) across all four cases, compared to that of the controls. (iii) By assembling these building blocks, we created an SMC patch (5 mm × 5 mm × 20 µm), which was cultured for 51 days forming a 3D tissue-like construct. The histology of the cultured patch was compared to that of a native rat bladder. These results indicate the potential of creating 3D tissue models at high throughput in vitro using building blocks. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2010 IOP Publishing Ltd |
| Название | A droplet-based building block approach for bladder smooth muscle cell (SMC) proliferation |
| Тип | paper |
| DOI | 10.1088/1758-5082/2/1/014105 |
| Electronic ISSN | 1758-5090 |
| Print ISSN | 1758-5082 |
| Журнал | Biofabrication |
| Том | 2 |
| Первая страница | 14105 |
| Последняя страница | 14113 |
| Аффилиация | Xu, F; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; |
| Аффилиация | Moon, S J; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; |
| Аффилиация | Emre, A E; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA |
| Аффилиация | Turali, E S; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA |
| Аффилиация | Song, Y S; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA |
| Аффилиация | Hacking, S A; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA |
| Аффилиация | Nagatomi, J; Department of Bioengineering, Clemson University, Clemson, SC, USA |
| Аффилиация | Demirci, U; Department of Medicine, Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA |
| Выпуск | 1 |
