| Автор | Iwami, K |
| Автор | Noda, T |
| Автор | Ishida, K |
| Автор | Morishima, K |
| Автор | Nakamura, M |
| Автор | Umeda, N |
| Дата выпуска | 2010-03-01 |
| dc.description | This paper reports a method for rapid prototyping of cell tissues, which is based on a system that extrudes, aspirates and refills a mixture of cells and thermoreversible hydrogel as a scaffold. In the extruding mode, a cell-mixed scaffold solution in the sol state is extruded from a cooled micronozzle into a temperature-controlled substrate, which keeps the scaffold in the gel state. In the aspiration mode, the opposite process is performed by Bernoulli suction. In the refilling mode, the solution is extruded into a groove created in the aspiration mode. The minimum width of extruded hydrogel pattern is 114 ± 15 µm by employing a nozzle of diameter 100 µm, and that of aspirated groove was 355 ± 10 µm using a 500 µm-diameter nozzle. Gum arabic is mixed with the scaffold solution to avoid peeling-off of the gel pattern from the substrate. Patterning of Sf-9 cell tissue is demonstrated, and the stability of the patterned cell is investigated. This system offers a procedure for rapid prototyping and local modification of cell scaffolds for tissue engineering. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2010 IOP Publishing Ltd |
| Название | Bio rapid prototyping by extruding/aspirating/refilling thermoreversible hydrogel |
| Тип | paper |
| DOI | 10.1088/1758-5082/2/1/014108 |
| Electronic ISSN | 1758-5090 |
| Print ISSN | 1758-5082 |
| Журнал | Biofabrication |
| Том | 2 |
| Первая страница | 14108 |
| Последняя страница | 14112 |
| Аффилиация | Iwami, K; Department of Mechanical Systems and Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan |
| Аффилиация | Noda, T; Department of Mechanical Systems and Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan |
| Аффилиация | Ishida, K; Department of Mechanical Systems and Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan |
| Аффилиация | Morishima, K; Department of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan |
| Аффилиация | Nakamura, M; Department of Life Sciences and Bioengineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan |
| Аффилиация | Umeda, N; Department of Mechanical Systems and Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan |
| Выпуск | 1 |
