| Автор | Gruene, M |
| Автор | Pflaum, M |
| Автор | Deiwick, A |
| Автор | Koch, L |
| Автор | Schlie, S |
| Автор | Unger, C |
| Автор | Wilhelmi, M |
| Автор | Haverich, A |
| Автор | Chichkov, B N |
| Дата выпуска | 2011-03-01 |
| dc.description | Laser-assisted bioprinting (LaBP) allows the realization of computer-generated 3D tissue grafts consisting of cells embedded in a hydrogel environment. In this study, human adipose-derived stem cells (hASCs) were printed in a free-scalable 3D grid pattern by means of LaBP. We demonstrate that neither the proliferation ability nor the differentiation behaviour of the stem cells was affected by the LaBP procedure. Furthermore, the 3D grafts were differentiated down the adipogenic lineage pathway for 10 days. We verify by quantitative assessments of adipogenic markers that the 3D grafts resemble cell lineages present in natural adipose tissue. Additionally, we provide the proof that even pre-differentiated hASCs could be utilized for the generation of 3D tissue grafts. These results indicate that the biofabrication of living grafts resembling their complex native origin is within reach. |
| Формат | application.pdf |
| Издатель | Institute of Physics Publishing |
| Копирайт | 2011 IOP Publishing Ltd |
| Название | Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells |
| Тип | paper |
| DOI | 10.1088/1758-5082/3/1/015005 |
| Electronic ISSN | 1758-5090 |
| Print ISSN | 1758-5082 |
| Журнал | Biofabrication |
| Том | 3 |
| Первая страница | 15005 |
| Последняя страница | 15013 |
| Аффилиация | Gruene, M; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Аффилиация | Pflaum, M; Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany |
| Аффилиация | Deiwick, A; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Аффилиация | Koch, L; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Аффилиация | Schlie, S; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Аффилиация | Unger, C; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Аффилиация | Wilhelmi, M; Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany |
| Аффилиация | Haverich, A; Medizinische Hochschule Hannover, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany |
| Аффилиация | Chichkov, B N; Nanotechnology Department, Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany |
| Выпуск | 1 |
