Synthesis, capping and binding of colloidal gold nanoparticles to proteins
Nghiem, Thi Ha Lien; La, Thi Huyen; Vu, Xuan Hoa; Chu, Viet Ha; Nguyen, Thanh Hai; Le, Quang Huan; Fort, Emmanuel; Do, Quang Hoa; Tran, Hong Nhung; Nghiem, Thi Ha Lien; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam; La, Thi Huyen; Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam; Vu, Xuan Hoa; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam; Chu, Viet Ha; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam; Nguyen, Thanh Hai; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam; Le, Quang Huan; Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam; Fort, Emmanuel; Institut Langevin, ESPCI Paris Tech, CNRS UMR 7587, 10 rue Vauquelin, 75231 Paris 05, France; Do, Quang Hoa; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam; Tran, Hong Nhung; Institute of Physics, Vietnam Academy of Science and Technology (VAST), 10 Dao Tan, Hanoi, Vietnam
Журнал:
Advances in Natural Sciences: Nanoscience and Nanotechnology
Дата:
2010-06-01
Аннотация:
Bovine serum albumin (BSA) was used as a stabilizing agent and biofunctionalized layer for water-dispersed gold nanoparticles (NPs) synthesized from metal precursor HAuCl<sub>4</sub>. The BSA binding to gold NPs was characterized qualitatively and quantitatively by transmission electron microscopy, UV-VIS and FTIR spectrophotometers. HER2 (human epidermal growth factor receptor 2) specific phage antibodies were attached to BSA stabilized gold NPs to form a gold–antibody complex. An ELISA (enzyme-linked immunosorbent assay) test was done to confirm the bioactivity of antibodies attached to gold NPs.
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