Detection of DNA of genetically modified maize by a silicon nanowire field-effect transistor
Pham, Van Binh; Tung Pham, Xuan Thanh; Duong Dang, Ngoc Thuy; Tuyen Le, Thi Thanh; Tran, Phu Duy; Nguyen, Thanh Chien; Nguyen, Van Quoc; Dang, Mau Chien; van Rijn, Cees J M; Tong, Duy Hien; Pham, Van Binh; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Tung Pham, Xuan Thanh; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Duong Dang, Ngoc Thuy; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Tuyen Le, Thi Thanh; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Tran, Phu Duy; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Nguyen, Thanh Chien; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Nguyen, Van Quoc; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Dang, Mau Chien; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; van Rijn, Cees J M; Nanosens Research B.V, Berkelkade 11, NL 7201 JE, Zutphen, The Netherlands; Tong, Duy Hien; Laboratory for Nanotechnology, Vietnam National University in Ho Chi Minh City, Community 6, Linh Trung Ward, Tu Duc District, Ho Chi Minh City, Vietnam; Nanosens Research B.V, Berkelkade 11, NL 7201 JE, Zutphen, The Netherlands
Журнал:
Advances in Natural Sciences: Nanoscience and Nanotechnology
Дата:
2011-06-01
Аннотация:
A silicon nanowire field-effect transistor based sensor (SiNW-FET) has been proved to be the most sensitive and powerful device for bio-detection applications. In this paper, SiNWs were first fabricated by using our recently developed deposition and etching under angle technique (DEA), then used to build up the complete SiNW device based biosensor. The fabricated SiNW biosensor was used to detect DNA of genetically modified maize. As the DNA of the genetically modified maize has particular DNA sequences of 35S promoter, we therefore designed 21 mer DNA oligonucleotides, which are used as a receptor to capture the transferred DNA of maize. In our work, the SiNW biosensor could detect DNA of genetically modified maize with concentrations down to about 200 pM.
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