A Rapid Method of preparing Pure Serum Gamma-Globulin
STANWORTH, D. R.
Журнал:
Nature
Дата:
1960
Аннотация:
IN the course of investigating various methods of isolating skin-sensitizing antibodies from human allergic serum, a procedure has been devised which could be applied to the rapid preparation of other 7S-type gamma-globulins in a high degree of purity. The technique employs diethyl amino ethyl cellulose anion exchanger, prepared according to the method used by Peterson and Sober<sup>1</sup>, to adsorb out of the serum all proteins other than 7S-type gamma-globulins. For this purpose, a batch process has proved superior to the more widely used column chromatography, because of its greater speed and ease of manipulation.
317.0Кб