| Автор | Grotjohann, Tim |
| Автор | Testa, Ilaria |
| Автор | Leutenegger, Marcel |
| Автор | Bock, Hannes |
| Автор | Urban, Nicolai T. |
| Автор | Lavoie-Cardinal, Flavie |
| Автор | Willig, Katrin I. |
| Автор | Eggeling, Christian |
| Автор | Jakobs, Stefan |
| Автор | Hell, Stefan W. |
| Дата выпуска | 2011 |
| dc.description | Lens-based optical microscopy failed to discern fluorescent features closer than 200â nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported fluorescence nanoscopy variants switch these features either with intense beams at defined positions or randomly, molecule by molecule. Here we demonstrate an optical nanoscopy that records raw data images from living cells and tissues with low levels of light. This advance has been facilitated by the generation of reversibly switchable enhanced green fluorescent protein (rsEGFP), a fluorescent protein that can be reversibly photoswitched more than a thousand times. Distributions of functional rsEGFP-fusion proteins in living bacteria and mammalian cells are imaged at <40-nanometre resolution. Dendritic spines in living brain slices are super-resolved with about a million times lower light intensities than before. The reversible switching also enables all-optical writing of features with subdiffraction size and spacings, which can be used for data storage. |
| Формат | application.pdf |
| Издатель | Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. |
| Копирайт | Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. |
| Название | Diffraction-unlimited all-optical imaging and writing with a photochromic GFP |
| Тип | ARTICLE |
| DOI | 10.1038/nature10497 |
| Print ISSN | 0028-0836 |
| Журнал | Nature |
| Том | 478 |
| Первая страница | 204 |
| Последняя страница | 208 |
| Аффилиация | Grotjohann, Tim; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Testa, Ilaria; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Leutenegger, Marcel; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Bock, Hannes; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Urban, Nicolai T.; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Lavoie-Cardinal, Flavie; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Willig, Katrin I.; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Eggeling, Christian; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Аффилиация | Jakobs, Stefan; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany; University of Göttingen Medical School, Robert-Koch-Str. 40, 37075 Göttingen, Germany |
| Аффилиация | Hell, Stefan W.; Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany |
| Выпуск | 7368 |
