Автор |
de Llano, D., González |
Автор |
Santa-María, G. |
Дата выпуска |
1997 |
dc.description |
Evolution of the whey proteins fractions and proteolysis indices from 12 batches of the artisan cheese Afuega'l Pitu variety were studied by reversed phase high performance liquid chro matography (RP-HPLC) over the ripening period. The correlation coefficients between the whey protein contents and ripening time were very low, indicating that the whey protein content did not change as the cheese aged. However, soluble nitrogen content and the percentage of total nitrogen in the soluble form showed correlation coefficients higher than 0.9 with increasing ripening time. Analysis of variance showed significant differences (p < 0.05) between dairies for most of the variables with the exception of β-lactoglobulin A and bovine serum albumin. These variables were also significantly different between the two manufacture zones. Conversely, no significant differences (p < 0.05) were observed between the two varieties of cheese, red and white, for whey protein content or for proteolysis indices. |
Издатель |
Sage Publications |
Тема |
Afuega'l Pitu cheese |
Тема |
whey |
Тема |
proteins |
Тема |
proteolysis |
Тема |
HPLC |
Название |
Analysis of whey proteins during ripening of Afuega'l Pitu cheese by reversed phase HPLC Análisis por RP-HPLC de las proteínas del suero del queso Afuega'l Pitu durante la maduración |
Тип |
Journal Article |
DOI |
10.1177/108201329700300606 |
Print ISSN |
1082-0132 |
Журнал |
Food Science and Technology International |
Том |
3 |
Первая страница |
445 |
Последняя страница |
449 |
Аффилиация |
de Llano, D., González, Insto de Productos Lácteos de Asturias (CSIC). PO Box 85, E-33300 Villaviciosa. Asturias. Spain |
Аффилиация |
Santa-María, G., Insto de Fermentaciones Industriales (CSIC). Juan de la Cierva 3. 28006 Madrid. Spain |
Выпуск |
6 |
Библиографическая ссылка |
Amigo L., Ibáñez I., Fernández E., Santa-María G. and Ramos M. (1989). Comparison of an electrophoretic and immunological method for the determination of goat and cow milk in cheese. Milchwissenschaft 44: 215-218. |
Библиографическая ссылка |
Andrews A.T. (1983). Proteinases in normal bovine milk and their action on caseins. Journal of Dairy Research 50: 45-55. |
Библиографическая ссылка |
Bütikofer U., Rüegg M. and Ardö Y. (1993). Determination of nitrogen fractions in cheese: evaluation of a collaborative study. Lebensmittel Wissenschaft und Technologie 26: 271-275. |
Библиографическая ссылка |
Cuesta P., Fernández-Garía E., González de Llano D., Montilla A. and Rodriguez A. (1996). Evolution of the microbiological and biochemical characteristics of Afuega'l Pitu cheese during ripening. Journal of Dairy Science 79: 1693-1698. |
Библиографическая ссылка |
De Frutos M., Cifuentes A., Diez-Masa J.C., Amigo L. and Ramos M. (1991). Application of HPLC for the detection of proteins in whey mixtures from different animal species. Journal of High Resolution Chromatography 14: 289-291. |
Библиографическая ссылка |
De Frutos M., Cifuentes A., Amigo L., Ramos M., Diez-Masa J.C. (1992). Rapid analysis of whey proteins from different animal species by reversed-phase high-performance liquid chromatography. Zeitschrift für Lebensmittel Untersuchung Forschung 195: 326-331. |
Библиографическая ссылка |
Di Luccia A., Ledda L., Moio L., Chianese L., Nicolai M.A., Spagna Musso S., Del Giovine L., Bocca A.and Addeo F. (1993). Quality control of Ricotta cheese based on protein composition. Italian Journal of Food Science 2: 168-183. |
Библиографическая ссылка |
Dixon W.J. (ed.) (1992). BMDP: Statistical Software Manual. Berkeley, CA: University of California Press. |
Библиографическая ссылка |
Gatti M., Locci F., Cattaneo T.M.P., Neviani E. and Rampilli M. (1994). Analisi della frazione sieroproteica in latti di specie diverse. Esame comparativo di metodi cromatografici FPLC ed HPLC . Scienza e Tecnica Lattiero Casearia 45: 233-246. |
Библиографическая ссылка |
González deLlano D., Polo C. and Ramos M. (1990). Update on HPLC and FPLC analysis of nitrogen compounds in dairy products. Lait 70: 255-277. |
Библиографическая ссылка |
González deLlano D., Polo C. and Ramos M. (1995). Study of proteolysis in artisanal cheeses: High performance liquid chromatography of peptides. Journal of Dairy Science 78: 1018-1024. |
Библиографическая ссылка |
Hillier R.M. (1976). The quantitative measurement of whey proteins using polyacrylamide-gel electrophoresis. Journal Dairy of Research 43: 259-265. |
Библиографическая ссылка |
International Dairy Federation (1964). Protein Content of Processed Cheese Products. IDF Standard 25. Brussels, Belgium: International. Dairy Federation . |
Библиографическая ссылка |
López-Gálvez G., Amigo L. and Ramos, M. (1994). Genetic polymorphism of whey proteins in two ovine breeds. Milchwissenschaft 49: 123-125. |
Библиографическая ссылка |
Molina E., Ramos M. and Martín-Alvarez P.J. (1995). Prediction of the percentages of cows', goats' and ewes' milk in "Iberico" cheese by electrophoretic analysis of whey proteins. Zeitschrift für Lebensmittel Untersustung und Forschung 201: 331-335. |
Библиографическая ссылка |
Pellegrino L., De Noni I., Tirelli A. and Resmini P. (1991). Determinazione del latte di vacca nei formaggi di specie minori mediante HPLC delle sieroproteine Nota 1: Applicazione alla Mozzarella di bufala. Scienza e Tecnica Lattiero Casearia 42: 87-101. |
Библиографическая ссылка |
Pellegrino L., Tirelli A. and Masotti F. (1992). Determinazione del latte di vacca nei formaggi di specie minori mediante HPLC delle sieroproteine Nota 2: Applicazione ai formaggi di pecora. Scienza e Tecnica Lattiero Casearia 43: 297-310. |
Библиографическая ссылка |
Recio I., Molina E., Ramos M. and de Frutos M. (1995). Quantitative analysis of major whey proteins by capillary electrophoresis using uncoated capillaries. Electrophoresis 16: 654-658. |
Библиографическая ссылка |
Resmini P., Pellegrino L., Andreini R. and Prati F. (1989a). Evaluation of milk whey proteins by reversed-phase HPLC. Scienza e Tecnica Lattiero Casearia 40: 7-19. |
Библиографическая ссылка |
Resmini P., Pellegrino L., Hogenboom J.A. and Andreini R. (1989b). Thermal denaturation of whey protein in pasteurized milk. Fast evaluation by HPLC. Italian Journal of Food Science 3: 51-62. |