| Автор | Portales-Pérez, D. |
| Автор | González-Amaro, R. |
| Автор | Abud-Mendoza, C. |
| Автор | Sánchez-Armáss, S. |
| Дата выпуска | 1997 |
| dc.description | Several immuno-regulatory abnormalities have been described in SLE patients. T cell dysfunction in SLE includes defective in vitro proliferative responses to several stimuli, reduced IL-2 production and a poor helper function. It has been widely proposed that this defective T cell immunoregulatory function has a key role in the hyperactivity of B cells and auto-antibody production in SLE. However, it has not been elucidated whether or not this cell dysfunction is intrinsic to lymphocytes or is due to other factors such as anti-lymphocyte auto-antibodies. In this study we have evaluated some important early cell activation events in T and non-T lymphocytes from patients with systemic lupus erythematosus (SLE).Peripheral blood lymphocytes from SLE patients and controls were isolated. The intracellular pH (pH<sub> i</sub>), cytosolic calcium (Ca<sup>2+</sup><sub>i</sub>) and CD69 expression were determined by spectrofluorometry and flow cytometry. Modifications of these parameters in response to protein kinase C (PKC) activators, mitogenic lectins and calcium ionophores were also studied. We found a significant reduction in the increase of pH<sub>i</sub> in response to PKC activators (PMA) in SLE cells. In addition, the induction of CD69 expression by PMA was significantly lower in T cells from SLE patients. By contrast, freshly isolated non-stimulated SLE cells exhibited a significantly higher pH<sub>i</sub>, as well as an increased baseline expression of the early cell activation antigen CD69. On the other hand, the increase in Ca<sup>2+</sup><sub>i</sub> in response to a Ca<sup> 2+</sup> ionophore (4Br-A23187) or thapsigargin in Ca<sup>2+</sup>-free solutions, was smaller in SLE lymphocytes. We concluded that T cells from SLE patients exhibit abnormalities in several key early cell activation events (pH<sub> i</sub>, Ca<sup>2+</sup><sub>i</sub> and CD69 expression). These abnormalities could have an important role in the T cell dysfunction observed in SLE. The presence of T cells with a preactivated phenotype in the peripheral blood of SLE patients, could be a reflection of the ongoing autoimmune phenomena that is occurring in these patients. |
| Издатель | Sage Publications |
| Тема | pHi |
| Тема | systemic lupus erythematosus |
| Тема | cell activation |
| Тема | Ca2+i |
| Тема | CD69 |
| Название | Abnormalities in CD69 expression, cytosolic pH and Ca2+ during activation of lymphocytes from patients with systemic lupus erythematosus |
| Тип | Journal Article |
| DOI | 10.1177/096120339700600107 |
| Print ISSN | 0961-2033 |
| Журнал | Lupus |
| Том | 6 |
| Первая страница | 48 |
| Последняя страница | 56 |
| Аффилиация | Portales-Pérez, D., Department of Immunology, Department of Physiology, School of Medicine, University of San Luis Potosi |
| Аффилиация | González-Amaro, R., Department of Immunology, Department of Physiology, School of Medicine, University of San Luis Potosi |
| Аффилиация | Abud-Mendoza, C., Hospital Central 'Dr Ignacio Morones Prieto', San Luis Potosi, S.L.P., México |
| Аффилиация | Sánchez-Armáss, S., Laboratory of Neuronal Physiology, Department of Physiology, School of Medicine, University of San Luis Potosi |
| Выпуск | 1 |
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