| Автор | Bronstein, J, M |
| Автор | Lallone, R |
| Автор | Seitz, R, S |
| Автор | Ellison, G, W |
| Автор | Myers, L, W |
| Дата выпуска | 1996 |
| dc.description | Oligodendrocyte-specifie protein (OSP) is a recently isolated novel protein found only in CNS laminar myelin and therefor is a good candidate as an autoantigen in patients with MS. In order to determine the humoral response in patients with MS. Westren blot analysis was performed on CSF from 6 patients with clinically stable relapsing MS (rMS) and 2 normal controls. All 6 of the CSF samples from rMS patients contained anti-OSP antibodies and none were detected in controls. Peptide mapping determined that the antigenic response was directed at a 7 amino acid peptide (OSP 114-120) which was 71% homologous with several common viral and bacterial proteins and 100% identical to the human OSP protein. ELISAs were performed using OSP 114-20 as antigen on a total 32 MS patints followed at UCLA, 53 MS patients from the National Neurological Rescarch Specimen Bank (NNRSB), and on 51 neurological control samples. Eighty % of UCLA rMS patients had an OSP ELISA reading above 0.55 OD units (median ± SD, 0.94 ± .35) while 0 of 14 CSF samples from HTLV-1 patients and normal controls had values above 0.55 units (0.28 ± .12; p<.01). Similar results were found in specimens from the NNRSB. No differences in anti-OSP titers were found in serum of MS and control patients. ELISAs performed on CSF samples using homologus viral peptieds as antigen (e.g. EBV, HSV HIV) showed a close correlation with anti-OSP 114-120 titers, and in some, the anti-viral titers far exceeded them. These data demonstrate a specific humoral response directed against a region of OSP in rMS patinets which cross reacts with several common viral peptides and suggests a possible role of molecular mimicry in the development of MS. |
| Издатель | SAGE Publications |
| Название | A humoral response to OSP in multiple sclerosis: A molecular mimic? |
| Тип | Journal Article |
| DOI | 10.1177/135245859600200512 |
| Electronic ISSN | 1477-0970 |
| Print ISSN | 1352-4585 |
| Журнал | Multiple Sclerosis |
| Том | 2 |
| Первая страница | 250 |
| Последняя страница | 250 |
| Аффилиация | Bronstein, J, M, UCLA School of Medicine, Department of Neurology, 710 Westwood Plaza, RNRC Los Angeles, CA 90024, USA |
| Аффилиация | Lallone, R, UCLA School of Medicine, Department of Neurology, 710 Westwood Plaza, RNRC Los Angeles, CA 90024, USA |
| Аффилиация | Seitz, R, S, UCLA School of Medicine, Department of Neurology, 710 Westwood Plaza, RNRC Los Angeles, CA 90024, USA |
| Аффилиация | Ellison, G, W, UCLA School of Medicine, Department of Neurology, 710 Westwood Plaza, RNRC Los Angeles, CA 90024, USA |
| Аффилиация | Myers, L, W, UCLA School of Medicine, Department of Neurology, 710 Westwood Plaza, RNRC Los Angeles, CA 90024, USA |
| Выпуск | 5 |
