| Автор | Lozeron, P |
| Автор | Chabas, D |
| Автор | Duprey, B |
| Автор | Lyon-Caen, O |
| Автор | Liblau, R |
| Дата выпуска | 1998 |
| dc.description | To better characterize the cellular immune response taking place in the MS central nervous system, we investigated the blood and CSF T cell receptor (TCR) Vβ5 and Vb17 repertoire in HLA-typed patients with recently diagnosed MS or other neurological diseases (OND). Using a RT-PCR based technique, we analysed directly ex vivo the CDR3 size of TCR β chains utilizing Vβ5 (eight patients with MS and one with OND) or Vβ17 (eight patients with MS and six with OND) gene segments on paired blood-CSF samples. Globally, the analysis of Vβ5-Jβ and Vβ17-Jβ repertoire showed a less diverse pattern in the CSF samples than in the corresponding peripheral blood lymphocytes both in MS and in OND patients. However, we did not detect any recurrent clonal expansion within the Vb5<sup>+</sup> T cells in MS patients, underlining the potential limits of Vβ5- based immunotherapy in MS. We found an expanded T cell population using the same Vβ17-Jβ1.6 combination with identical CDR3 length in the CSF of three MS patients and none of the control patients. These results suggest selective expansion of T cells expressing this segment gene in the MS central nervous system. |
| Издатель | Sage Publications |
| Тема | multiple sclerosis |
| Тема | cerebrospinal fluid |
| Тема | T cell |
| Тема | T cell receptor |
| Тема | autoimmunity |
| Тема | immunotherapy |
| Название | T cell receptor Vb5 and Vb17 clonal diversity in cerebrospinal fluid and peripheral blood lymphocytes of multiple sclerosis patients |
| Тип | Journal Article |
| DOI | 10.1177/135245859800400313 |
| Print ISSN | 1352-4585 |
| Журнал | Multiple Sclerosis |
| Том | 4 |
| Первая страница | 154 |
| Последняя страница | 161 |
| Аффилиация | Lozeron, P, Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France |
| Аффилиация | Chabas, D, Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France |
| Аффилиация | Duprey, B, Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France |
| Аффилиация | Lyon-Caen, O, Fédération de Neurologie, Hopital de la Salpêtrière, 75651 Paris cedex 13, France |
| Аффилиация | Liblau, R, Cellular Immunology Laboratory, Hopital de la Salpêtrière, 75651 Paris cedex 13, France, INSERM CJF 9608, Hopital de la Salpêtrière, 75651 Paris cedex 13, France |
| Выпуск | 3 |
| Библиографическая ссылка | Ebers GC, Sadovnick AD. (1997) Susceptibility: genetics in multiple sclerosis. In: Paty DW, Ebers GC (eds). Multiple sclerosis. FA Davis Company: Philadelphia pp. 29-47. |
| Библиографическая ссылка | Sibley WA, Bamford CR, Laguna JF. (1985) Clinical viral infections and multiple sclerosis. Lancet 1: 1313-1315. |
| Библиографическая ссылка | Wekerle Het al. (1994) Animal models. Ann Neurol 36: S47-S53. |
| Библиографическая ссылка | Traugott U, Reinhertz EL, Raine CS. (1983) Multiple sclerosis: distribution of T cells, T cell subsets and Ia-positive macrophages in lesions of different ages. J Neuroimmunol 4: 201-221. |
| Библиографическая ссылка | Olerup O, Hillert J. (1991) HLA class II associated genetic susceptibility in multiple sclerosis: A critical evaluation. Tissue Antigens 38: 1-15. |
| Библиографическая ссылка | Toyanaga Bet al. (1985) Organisation and sequences of the diversity, joining, and constant region genes of the human T cell receptor β chain. Proc Natl Acad Sci USA 82: 8624-8628. |
| Библиографическая ссылка | Pannetier Cet al. (1993) The size of the CDR3 hypervariable regions of the murine T-cell receptor β chains vary as a function of the recombined germline segments. Proc Natl Acad Sci USA 90: 4319-4323. |
| Библиографическая ссылка | Garboczi Det al. (1996) Structure of the complex between human T-cell receptor, viral peptide and HLA-A2. Nature 384: 134-141. |
| Библиографическая ссылка | Acha-Orbea Het al. (1988) Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention. Cell 54: 263-273. |
| Библиографическая ссылка | Offner Het al. (1993)Where, when and how to detect biased expression of disease relevant Vβ genes in rats with experimental autoimmune encephalomyelitis. J Immunol 151: 506-517. |
| Библиографическая ссылка | Gold DPet al. (1991) Analysis of T cell receptor β chains in Lewis rats with experimental allergic encephalomyelitis: conserved complementary determining region 3. J Exp Med 174: 1467-1476. |
| Библиографическая ссылка | Howell MDet al. (1989) Vaccination against experimental allergic encephalomyelitis with T cell receptor peptides. Science 246: 668-670. |
| Библиографическая ссылка | Vandenbark A, Hashim G, Offner H. (1989) Immunisation with a synthetic T cell receptor V region peptide protects against experimental autoimmune encephalomyelitis. Nature 341: 541-543. |
| Библиографическая ссылка | Ben-Nun Aet al. (1991) Restricted T-cell receptor Vβ gene usage by myelin basic protein-specific T-cell clones in multiple sclerosis: predominant genes vary in individuals. Proc Natl Acad Sci USA 88: 2466-2470. |
| Библиографическая ссылка | Richert JRet al. (1995) Diversity of T cell receptor Vα, Vβ and CDR3 expression by myelin basic protein specific human T cell clones. Neurology 45: 1919-1922. |
| Библиографическая ссылка | Chou YKet al. (1994) T cell receptor Vβ gene usage in the recognition of myelin basic protein by cerebrospinal fluid- and blood-derived T cells from patients with multiple sclerosis. J Neurosci Res 37: 169-181. |
| Библиографическая ссылка | Vandevyver Cet al. (1995) Clonal expansion of myelin basic protein-reactive T cells in patients with multiple sclerosis: restricted T cell receptor V gene rearrangements and CDR3 sequence. Eur J Immunol 25: 958-968. |
| Библиографическая ссылка | Hafler Aet al. (1996) TCR usage in human and experimental demyelinating disease. Immunol Today 17: 152-159. |
| Библиографическая ссылка | Martin Ret al. (1992) Diversity in fine specificity and T cell receptor usage of the human CD4+ cytotoxic T cell response specific for the immunodominant myelin basic protein peptide 87-106. J Immunol 148: 1359-1366. |
| Библиографическая ссылка | Kotzin Let al. (1991) Preferential T-cell receptor β chain variable gene use in myelin basic protein-reactive T-cell clones from patients with multiple sclerosis. Proc Natl Acad Sci USA 88: 9161-9165. |
| Библиографическая ссылка | Wucherpfennig Ket al. (1990) Shared human T cell receptor Vβ usage to immunodominant regions of myelin basic protein. Science 248: 1016-1018. |
| Библиографическая ссылка | Oksenberg Jet al. (1993) Selection for T-cell receptor Vβ-Dβ-Jβ rearrangements with specificity for a myelin basic protein peptide in brain lesions of multiple sclerosis. Nature 362: 68-70. |
| Библиографическая ссылка | Musette Pet al. (1996) Expansion of a recurrent Vβ5.3+ T-cell population in newly diagnosed untreated HLA-DR2 multiple sclerosis patients. Proc Natl Acad Sci USA 93: 12461-12466. |
| Библиографическая ссылка | Vandenbark Aet al. (1996) Treatment of multiple sclerosis with T cell receptor peptides: results of a double blind pilot trial. Nature Medicine 2: 1109-1115. |
| Библиографическая ссылка | Gold Det al. (1997) Results of a phase I clinical trial of a T cell receptor vaccine in patients with multiple sclerosis. II Comparative analysis of TCR utilisation in CSF T cell populations before and after vaccination with a TCR Vβ6 CDR2 peptide. J Neuroimmunol 76: 29-38. |
| Библиографическая ссылка | Buenafe Aet al. (1994) A TCR Vα CDR3 specific motif associated with Lewis rat autoimmune encephalomyelitis and basis protein-specific T cell clones. J Immunol 153: 386-394. |
| Библиографическая ссылка | Lee Set al. (1991) Common T cell receptor Vβ usage in oligoclonal T lymphocytes derived from cerebrospinal fluid and blood of patients with multiple sclerosis. Ann Neurol 29: 33-40. |
| Библиографическая ссылка | Birnbaum G, Ness B. (1992) Quantitation of T cell receptor Vβ chain expression on lymphocytes from blood, brain and spinal fluid in patients with multiple sclerosis and other neurological diseases. Ann Neurol 32: 24-30. |
| Библиографическая ссылка | Usuku Ket al. (1996) Biased expression of T cell receptor genes characterizes activated T cells in multiple sclerosis cerebrospinal fluid. J Neurosci Res 45: 829-837. |
| Библиографическая ссылка | Wilson DBet al. (1997) Results of a phase I clinical trial of a T-cell receptor peptide vaccine in patients with multiple sclerosis. I Analysis of T cell receptor utilization in CSF cell populations. J Neuroimmunol 76: 15-28. |
| Библиографическая ссылка | Poser CMet al. (1983) New diagnostic criteria for multiple sclerosis: guidelines for research protocols. Ann Neurol 13: 227-231. |
| Библиографическая ссылка | Chomczynski P, Sacchi N. (1987) Single method of RNA isolation by acid guanidium isocyanate phenol chloroform extraction. Ann Biochem 162: 156-159. |
| Библиографическая ссылка | Genevée Cet al. (1992) An experimentally validated panel of subfamily-specific oligonucleotide primers (Vα1-w29/Vβ1-w24) for the study of human T cell receptor variable V gene segment usage by polymerase chain reaction. Eur J Immunol 22: 1261-1269. |
| Библиографическая ссылка | Pannetier Cet al. (1995) T-cell repertoire diversity and clonal expansions in normal and clinical samples. Immunol Today 16: 176-181. |
| Библиографическая ссылка | Hafler DAet al. (1985) In vivo activated T lymphocytes in the peripheral blood and cerebrospinal fluid of patients with multiple sclerosis. N Engl J Med 312: 1405-1411. |
| Библиографическая ссылка | Noronha A, Richman DP, Arnason BGW (1985) Multiple sclerosis: activated cells in cerebrospinal fluid in acute exacerbations. Ann Neurol 18: 722-725. |
| Библиографическая ссылка | Svenningsson Aet al. (1993) Adhesion molecules expression on cerebrospinal fluid T lymphocytes: evidence for common recruitment mechanisms in multiple sclerosis, aseptic meningitis, and normal controls. Ann Neurol 34: 155-161. |
| Библиографическая ссылка | Oksaranta Oet al. (1995) Influx of nonactivated T lymphocytes into the cerebrospinal fluid during relapse of multiple sclerosis. Ann Neurol 38: 465-468. |
| Библиографическая ссылка | Wucherpfennig Ket al. (1992) T cell receptor Vα-Vβ receptoire and cytokine gene expression in active multiple sclerosis lesions. J Exp Med 175: 993-1002. |
| Библиографическая ссылка | Rowen L, Koop BF, Hood L. (1996) The complete 685- kilobase DNA sequence of the human β T cell receptor locus. Science 272: 1755-1762. |
| Библиографическая ссылка | Perronet al. (1997) Molecular identification of a novel retrovirus repeatedly isolated from patients with multiple sclerosis. Proc Natl Acad Sci USA 94: 7583-7588. |
