| Автор | Jr, Gilbert, R Upchurch |
| Автор | Loscalzo, Joseph |
| Автор | Banes, Albert, J |
| Дата выпуска | 1997 |
| dc.description | Several physical factors, including shear stress and cyclic load, modulate the ability of endothelial cells to respond to injury. The objective of these experiments was to test the hypothesis that cyclic mechanical load stimulates endothelial cell DNA synthesis and division in vitro. Rabbit aortic endothelial cells were cultured on Flex® flexible-bottomed culture plates, and subjected to load amplitudes of increasing magnitude (0, 0.18, 0.24 and 0.27 load at 1 Hz) using a Flexercell® strain unit. Cells were harvested enzymatically and cell numbers determined on days 1, 3 and 5 after initiating the load regimen. DNA synthesis was quantified after trichloroacetic acid precipitation of [<sup>3</sup>H]thymidine-labeled cells from: (1) whole culture wells and (2) areas of minimum and maximum strain in culture cells. Data were analyzed using analysis of variance and a Tukeyʼs test (n = 6 observations/strain regimen per day in triplicate). Results from analysis of endothelial cells in whole, subconfluent cultures showed that cells subjected to strains of 0.18 had a decreased rate of cell division (76% of control) and DNA synthesis (63% of control), while cells subjected to strains of 0.24 and 0.27 had an increased rate of cell division (108 and 83% increase, respectively, compared with control; p < 0.001) and DNA synthesis (39 and 172% increase, respectively, compared with control; p < 0.001 for 0.27) on day 3 when compared with control cells. The results indicate that endothelial cells respond to various physiologic levels of cyclic load in a biphasic manner to initiate DNA synthesis and cell division. These data suggest that endothelial cell mitogenesis may be modulated by specific levels of cyclic load. |
| Издатель | SAGE Publications |
| Тема | cyclic load |
| Тема | DNA synthesis |
| Тема | endothelial cell |
| Название | Changes in the Amplitude of Cyclic Load Biphasically Modulate Endothelial Cell DNA Synthesis and Division |
| Тип | Journal Article |
| DOI | 10.1177/1358863X9700200104 |
| Electronic ISSN | 1477-0377 |
| Print ISSN | 1358-863X |
| Журнал | Vascular Medicine |
| Том | 2 |
| Первая страница | 19 |
| Последняя страница | 24 |
| Аффилиация | Jr, Gilbert, R Upchurch, Department of Surgery, Brigham and Womenʼs Hospital, Harvard University, NC, USA |
| Аффилиация | Loscalzo, Joseph, Whitaker Cardiovascular Institute, and Evans Department of Medicine, Boston University School of Medicine, NC, USA |
| Аффилиация | Banes, Albert, J, Department of Surgery, University of North Carolina at Chapel Hill, NC, USA |
| Выпуск | 1 |
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