Il metabolismo degli ormoni sessuali da parte degli Anfibi: Ricerche in vitro e in vivo
Chieffi, Giovanni; Chieffi, Giovanni; Istituto di Genetica della Università di Napoli
Журнал:
Bolletino di zoologia
Дата:
1956
Аннотация:
SummaryThe numerous researches on the effect of steroid hormones on the sex differentiation in Amphibia have shown different kinds of reaction according to the quality and the dose of the hormone used and according to the group of animals considered.The disagreement between the effects observed in the various groups of animals and the effects achieved varying the dose of the hormone have led to several hypotheses.The aim of the present work is to test one of these hypotheses namely the metabolic one.The experiments were carried out in vivo and, in the case of testosterone, also in vitro. As we know, testosterone masculinizes the gonads of the genus Rana, does not affect those of the genera Discoglossus, Bufo Alytes and Xenopus, and feminizes the larvae of the genera Ambystoma and Pleurodeles. Therefore it has been studied the metabolism of testosterone in vitro by liver slices of larvae, newly metamorphosed larvae and adults of different species of Amphibia (Rana pipiens, Rana catesbiana, Xenopus laevis, Ambystoma maculatum, Ambystoma opacum). Significant differences were found between different species and between different stages of development.The experiments on the metabolism of testosterone and estradiol in vivo (in collaboration with Dr. C. Y. Chang) were carried out with larvae of Rana pipiens, Rana catesbiana, Rana sylvatica, Xenopus laevis and Ambystoma opacum. Even in this case interesting differences were found among species.The metabolism in vivo is discussed and compared, in the case of testosterone, with that observed in vitro.
336.7Кб