Capillary zone electrophoresis of human recombinant erythropoietin using C8 coated columns without additives in the running buffer

Abstract

The use of C8 coated capillary columns enabled the characterization of human recombinant erythropoietin (r-HuEPO) as well as its determination by capillary electrophoresis. Samples were injected at 90 V cm1 during a period at 30 s and the electrophoregrams were recorded at 210 V cm1. A 0.03 mol l1 phosphate solution at pH 7.0 (without additives) was used as the running buffer. A minimum amount of 19 1 pg, which corresponds to a concentration in the sample of 0.37 g ml1, was found using UV detection at 240 nm. Several experiments were carried out in order to study the behaviour of r-HuEPO in this type of capillary. r-HuEPO was determined in spiked urine samples.