Автор |
Focarelli, Riccardo |
Автор |
Giuffrida, Andrea |
Автор |
Rosati, Floriana |
Дата выпуска |
1995 |
dc.description |
Changes in the distribution of sialylglycoconjugates on the surface of uncapacitated and in-vitro capacitated human spermatozoa were studied by means of two sialic acid specific lectins [Maackia amurensis agglutinin and Sambucus nigra agglutinin). On the uncapacitated sperm surface, sialylglycoconjugates were found be localized from the post-acrosomal region of the sperm head to the tail middle piece, whereas after invitro capacitation these molecules were only found in a small area of the post-acrosomal region. The surface of capacitated human spermatozoa was also investigated by specifically radiolabelling its terminal sialic acid residues. A 20 kDa glycoprotein, which was partially purified by anion-exchange chromatography, was the main component of the sialylglycoconjugate pattern after in-vitro capacitation. |
Формат |
application.pdf |
Издатель |
Oxford University Press |
Копирайт |
© Oxford University Press |
Тема |
capacitation |
Тема |
glycoconjugates |
Тема |
human spermatozoa |
Тема |
lectins |
Тема |
sialic acid |
Тема |
Article |
Название |
Changes in the sialylglycoconjugate distribution on the human sperm surface during in-vitro capacitation: partial purification of a 20 kDa sialylglycoprotein of capacitated spermatozoa |
Тип |
research-article |
Electronic ISSN |
1460-2350 |
Print ISSN |
0268-1161 |
Журнал |
Human Reproduction |
Том |
10 |
Первая страница |
2755 |
Последняя страница |
2759 |
Аффилиация |
Department of Evolutionary Biology, University of SienaVia Mattioli 4 53100 Siena, Italy |
Выпуск |
10 |