| Автор | Landy, Arthur |
| Автор | Foeller, Carl |
| Автор | Reszelbach, Rosalie |
| Автор | Dudock, Bernard |
| Дата выпуска | 1976 |
| dc.description | SummaryHigh pressure liquid chromatography on the RPC-5 reversed-phase ion exchange system has been shown to have several potential applications as an initial high capacity step in the isolation of specific DNA restriction fragments. The fractionation of a Hinc II digest of λ DNA, which contains 35 fragments with “flush ends” ranging in size from 3 × 10<sup>6</sup> to 7 × 10<sup>4</sup> daltons, has been used as a model system. Under certain conditions there are some restriction fragments whose elution relative to other fragments is different on RPC-5 chromatography than it is on gel electrophoresis. In some special circumstances it is possible to obtain satisfactory yields (60–70%) of a pure restriction fragment after a single passage through an RPC-5 column. |
| Формат | application.pdf |
| Издатель | Oxford University Press |
| Копирайт | © 1976 Information Retrieval Limited |
| Тема | Articles |
| Название | Preparative fractionation of DNA restriction fragments by high pressure column chromatography on RPC-5 |
| Тип | research-article |
| DOI | 10.1093/nar/3.10.2575 |
| Electronic ISSN | 1362-4962 |
| Print ISSN | 0305-1048 |
| Журнал | Nucleic Acids Research |
| Том | 3 |
| Первая страница | 2575 |
| Последняя страница | 2592 |
| Аффилиация | Landy Arthur; Division of Biology and Medicine, Brown University |
| Аффилиация | Foeller Carl; Division of Biology and Medicine, Brown University |
| Аффилиация | Reszelbach Rosalie; Department of Biochemistry, SUNY at Stony Brook |
| Аффилиация | Dudock Bernard; Department of Biochemistry, SUNY at Stony Brook |
| Выпуск | 10 |
